An atypical RNA silencing suppression strategy provides a snapshot of the evolution of sweet potato-infecting potyviruses

Plant viruses usually encode proteins with RNA silencing suppression (RSS) activity to counteract plant defenses. In Potyvirus , the largest genus in the family Potyviridae , this role is taken over by the multifunctional HCPro, also involved in aphid transmission, polyprotein processing and virion...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2018-10, Vol.8 (1), p.15937-10, Article 15937
Main Authors: Rodamilans, Bernardo, Valli, Adrián, Mingot, Ares, San León, David, López-Moya, Juan José, García, Juan Antonio
Format: Article
Language:English
Subjects:
38/35
38/44
38/70
38/77
631/326/596/2557
631/449/2661/2666
82/1
82/29
82/80
Humanities and Social Sciences
Ipomoea batatas
multidisciplinary
Plant virus diseases
Plant viruses
Plum pox
Potatoes
Potyvirus
RNA-mediated interference
Science
Science (multidisciplinary)
Viral infections
Virions
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant viruses usually encode proteins with RNA silencing suppression (RSS) activity to counteract plant defenses. In Potyvirus , the largest genus in the family Potyviridae , this role is taken over by the multifunctional HCPro, also involved in aphid transmission, polyprotein processing and virion formation. Recently, the large P1 of Sweet potato feathery mottle virus (SPFMV) was characterized finding an extra ORF produced after polymerase slippage, which originates the product P1N-PISPO. Transient expression assays showed that SPFMV P1 and P1N-PISPO presented RSS activity, while HCPro did not. In this work, we analyze possible differences between HCPro of SPFMV and other potyviruses, testing HCPro RSS activity in a transient expression assay, and using a Plum pox virus -based system to test the ability of SPFMV P1N-PISPO and HCPro to serve as RNA silencing suppressors in the context of a viral infection. Our results indicate that not only P1 and P1N-PISPO, but also HCPro display RSS activity when expressed in a suitable context, stressing the importance of the selected experimental system for testing anti-silencing capacity of proteins. The presence of multiple viral silencing suppressors in SPFMV adds complexity to an already intricate RSS system, and provides insight into the hypothetical evolution of sweet potato-infecting potyvirids.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-34358-y