Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat

• Published in: Oxidative medicine and cellular longevity 2018-01, Vol.2018 (2018), p.1-22
• Main Authors: Chen, Yung-Lung, Chung, Sheng-Ying, Wang, Ching-Jen, Lee, Mel S., Yip, Hon-Kan, Sheu, Jiunn-Jye, Sun, Cheuk-Kwan, Lee, Fan-Yen, Shao, Pei-Lin, Chen, Kuan-Hung, Wallace, Christopher Glenn, Yin, Tsung-Cheng, Sung, Pei-Hsun, Omran, Mostafa Mohammad
• Format: Article
• Language: English
• Subjects: Adipose Tissue - metabolism; Angiogenesis; Animals; Apoptosis; Apoptosis - drug effects; Biomarkers - metabolism; Cell culture; Cell Line; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Coculture Techniques; Collagen; Collagen - metabolism; Coronary vessels; Cytochrome c; DNA Damage; Echocardiography; Ejection fraction; Endothelial Progenitor Cells - drug effects; Endothelial Progenitor Cells - metabolism; Extracorporeal Shockwave Therapy; Gene expression; Heart attack; Heart attacks; Ischemia; Laboratory animals; Male; Melatonin; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Mitochondrial Dynamics - drug effects; Myocardial Infarction - diagnostic imaging; Myocardial Infarction - pathology; Myocardial Infarction - physiopathology; Ostomy; Oxidative Stress - drug effects; Povidone; Rats, Sprague-Dawley; Rodents; Stem cells; Stromal Cells - drug effects; Stromal Cells - metabolism; Time Factors; Tumor Suppressor p53-Binding Protein 1 - metabolism; Veins & arteries; Ventricular Function, Left - drug effects; Ventricular Function, Left - physiology; Ventricular Remodeling - drug effects; Ventricular Remodeling - physiology; Vitamin K 3 - pharmacology; X-ray Repair Cross Complementing Protein 1 - metabolism
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2018/7518920

This study tested the hypothesis that extracorporeal shock wave- (ECSW-) assisted adipose-derived stromal vascular fraction (SVF) therapy could preserve left ventricular ejection fraction (LVEF) and inhibit LV remodeling in a rat after acute myocardial infarction (AMI). Adult male SD rats were categorized into group 1 (sham control), group 2 (AMI induced by left coronary artery ligation), group 3 [AMI + ECSW (280 impulses at 0.1 mJ/mm2, applied to the chest wall at 3 h, days 3 and 7 after AMI), group 4 [AMI + SVF (1.2 × 106) implanted into the infarct area at 3 h after AMI], and group 5 (AMI + ECSW-SVF). In vitro, SVF protected H9C2 cells against menadione-induced mitochondrial damage and increased fluorescent intensity of mitochondria in nuclei (p