Molecular diversity assessment of a world collection of safflower genotypes by SRAP and SCoT molecular markers

Safflower ( Carthamus tinctorius L.) is considered as an oil crop that is rich in medicinal and industrial properties. In this study, the genetic diversity of safflower was assessed using 12 polymorphic sequence-related amplified polymorphism (SRAPs) and 11 polymorphic start codon targeted (SCoT) ma...

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Bibliographic Details
Published in:Physiology and molecular biology of plants 2018-11, Vol.24 (6), p.1261-1271
Main Authors: Golkar, Pooran, Mokhtari, Niloofar
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Carthamus tinctorius
Cell Biology
Conservation genetics
Gene polymorphism
Genetic diversity
Genetics
Genotypes
Life Sciences
Markers
Plant Physiology
Plant Sciences
Polymorphism
Population genetics
Primers
Research Article
Variance analysis
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Summary:Safflower ( Carthamus tinctorius L.) is considered as an oil crop that is rich in medicinal and industrial properties. In this study, the genetic diversity of safflower was assessed using 12 polymorphic sequence-related amplified polymorphism (SRAPs) and 11 polymorphic start codon targeted (SCoT) markers in 100 genotypes of safflower gathered from different geographical regions of the world. The 23 primers generated a total of 227 polymorphism fragments with a mean of 68.2% within the range of 3 (SCoT 31 and SCoT 35) to 13 (SCoT 35) bands per primer. Polymorphism per primer ranged between 100% (in Me4–Em1) and 18.1% (in SCoT19), with an average of 36.76%. The polymorphism information contents of the SRAP and SCoT markers were 0.35 and 0.30, respectively, indicating that SRAP markers were more effective than SCoT markers for assessing the degree of genetic diversity of the safflower. The results of the analysis of molecular variance showed a significant difference across cultivated safflower genotypes possessing a high intra-population variation. The examined accessions were categorized into five clusters based on similarity centers: the Middle East containing Iran, Iraq, Turkey, and Tajikistan; the Far East, including India, Pakistan, and Korea; Europe; the American continent; and Africa, including Egypt, Sudan and Libya. The present study shows the effectiveness of employing the mixture of SRAP and SCoT markers in the identification of safflower genetic diversity that would be useful for conservation and population genetics of safflower improvement in further studies.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-018-0545-0