Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens

• Published in: Oxidative medicine and cellular longevity 2018-01, Vol.2018 (2018), p.1-10
• Main Authors: Bao, Endong, Xu, Jiao, Yin, Bin, Tang, Shu
• Format: Article
• Language: English
• Subjects: Acids; alpha-Crystallin B Chain - metabolism; Animals; Antioxidants; Apoptosis; Biochemistry; Chickens - metabolism; Chromatography, High Pressure Liquid; Creatine kinase; Emulsions - chemistry; Food; Heart failure; Heat; Heat shock proteins; Heat-Shock Response - drug effects; Heatstroke; HSP70 Heat-Shock Proteins - metabolism; Immunohistochemistry; Kinases; Medical research; Metabolism; Metabolites; Myocardium - enzymology; Myocardium - pathology; Nanoparticles - chemistry; Nutrition; Oxidation; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Poultry; Poultry industry; Proteins; Rosmarinus - chemistry
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2018/7014126

Heat stress negatively affects poultry production and animal health. In response, animals invoke a heat stress response by inducing heat shock proteins (HSPs). Scientists are actively seeking natural products that can enhance the heat shock response. The present study aimed at assessing the effects of a purified rosemary extract comprising antioxidant compounds on the heat shock response and HSP expression profile in broiler chickens. The response of broilers to HS in the presence of purified rosemary extract was assessed using an in vivo myocardial cell model. Pathological lesions of heart tissue were examined microscopically. The levels and activities of enzymes associated with heart damage and oxidative damage were detected. Immunohistochemical staining was performed for HSPs in myocardial cells. The results showed that lactate dehydrogenase (LDH), creatine kinase (CK), and myocardial CK (CKMB) levels were reduced by the purified rosemary extract before and during heat stress. Heat stress alone increased CK and CKMB levels. The levels of oxidative damage-associated enzymes were compared between the rosemary + heat stress and heat stress-alone groups. The results indicated that in terms of these enzymes, the purified rosemary extract induced a more antioxidative state. Pathological examinations showed that heat stress caused myocardial fiber fracture, karyopyknosis, and degeneration. The addition of purified rosemary extract ameliorated these lesions to some degree, preserving more of the basic structure. Heat stress decreased the cellular levels of crystallin alpha B (CRYAB) and HSP70. The addition of the purified rosemary extract significantly increased the levels of CRYAB and HSP70 during heat stress (p