TRPV2 is required for mechanical nociception and the stretch-evoked response of primary sensory neurons

Mechanotransduction plays important roles in many sensory processes, including touch, pain, hearing, and proprioception. However, the molecular mechanisms of mechanical nociception have remained unclear. Here, we showed that elimination of transient receptor potential vanilloid 2 (TRPV2) in mice res...

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Bibliographic Details
Published in:Scientific reports 2018-11, Vol.8 (1), p.16782-10, Article 16782
Main Authors: Katanosaka, Kimiaki, Takatsu, Satomi, Mizumura, Kazue, Naruse, Keiji, Katanosaka, Yuki
Format: Article
Language:English
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Calcium
Decay
Humanities and Social Sciences
Mechanical properties
Mechanical stimuli
Mechanotransduction
Molecular modelling
multidisciplinary
Pain perception
Proprioception
Rodents
Science
Science (multidisciplinary)
Sensory neurons
Somatosensory system
Tactile stimuli
Transient receptor potential proteins
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Summary:Mechanotransduction plays important roles in many sensory processes, including touch, pain, hearing, and proprioception. However, the molecular mechanisms of mechanical nociception have remained unclear. Here, we showed that elimination of transient receptor potential vanilloid 2 (TRPV2) in mice resulted in the deficit of mechanical nociception due to the lack of mechanosensitivity in a subclass of adult primary sensory neurons (PSNs). The PSN-specific TRPV2-deficient mice showed behavioural impairment of mechanical nociception in tail-pressure and von Frey hair tests, without defects in axonal growth and neuronal composition. Conversely, the mice displayed normal behaviour to noxious heat and non-noxious tactile stimuli. Furthermore, based on the stretch-evoked Ca 2+ response of cultured PSNs, we characterised two types of stretch-activated neurons in normal mice; fast-decay high-threshold and slow-decay low-threshold mechanosensitive. The cultured neurons from TRPV2-deficient mice lacked stretch-evoked Ca 2+ responses by fast-decay neurons normally activated by high-threshold mechanical stimulation. These results demonstrated that TRPV2 has a critical role in mechanical nociception in the adult somatosensory system.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-35049-4