A novel dry-bonding approach to reduce collagen degradation and optimize resin-dentin interfaces

In dentistry, the wet-bonding approach relies on water to maintain demineralized collagen expanded for proper resin infiltration; nevertheless, hydrolytic instability of the resin-dentin interface is inevitable with current bonding techniques. Considering dimethyl sulfoxide’s (DMSO) ability to “biom...

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Bibliographic Details
Published in:Scientific reports 2018-11, Vol.8 (1), p.16890-8, Article 16890
Main Authors: Stape, Thiago Henrique Scarabello, Seseogullari-Dirihan, Roda, Tjäderhane, Leo, Abuna, Gabriel, Martins, Luís Roberto Marcondes, Tezvergil-Mutluay, Arzu
Format: Article
Language:English
Subjects:
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Biochemical analysis
Bond strength
Collagen
Dentin
Dentistry
Dimethyl sulfoxide
Enzymes
Gelatinase A
Gelatinase B
Humanities and Social Sciences
Hybridization
Hydroxyproline
Interfaces
Molars
multidisciplinary
Peptides
Resins
Science
Science (multidisciplinary)
Slabs
Teeth
Water content
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Summary:In dentistry, the wet-bonding approach relies on water to maintain demineralized collagen expanded for proper resin infiltration; nevertheless, hydrolytic instability of the resin-dentin interface is inevitable with current bonding techniques. Considering dimethyl sulfoxide’s (DMSO) ability to “biomodify” collagen and precipitate enzymes, the aim was to test whether the use of DMSO would permit adequate resin bonding to H 3 PO 4 -etched dehydrated dentin and assess its impact on collagen degradation by host-derived enzymes. Etched dentin surfaces from extracted sound human molars were randomly bonded in wet or dry conditions using aqueous or ethanolic DMSO solutions as pretreatments and bonding resins with or without DMSO. Bonded teeth were sectioned into resin-dentin slabs for confocal in situ zymography and beams for microtensile bond strength test. Demineralized powdered dentin was incubated in the tested DMSO -media and a hydroxyproline assay evaluated dissolution of collagen peptides. Zymography was performed on protein extracts obtained from dry and wet H 3 PO 4 -ecthed dentin powder treated with the DMSO- media. The correlative biochemical analysis demonstrated that reduction of water content during dentin hybridization by the innovative dry-bonding approaches with DMSO is effective to inactivate host-derived MMP-2 and MMP-9 and thus reduce collagen degradation while simultaneously optimizing resin-dentin bonding.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-34726-8