Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples

A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/m...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2018-11, Vol.19 (11), p.871-883
Main Authors: Zhang, Xian, He, Ke, Fang, Yun, Cao, Tong, Paudyal, Narayan, Zhang, Xiao-feng, Song, Hou-hui, Li, Xiao-liang, Fang, Wei-huan
Format: Article
Language:English
Subjects:
Animal Feed
Biomedical and Life Sciences
Biomedicine
Calibration
Chromatography, Affinity
Chromatography, Liquid
Coefficient of variation
Colloids
Corn
Food Contamination - analysis
Food Safety
Gold
Immunoassay - methods
Inhibitory Concentration 50
Limit of Detection
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metal Nanoparticles
Ochratoxin A
Ochratoxins - analysis
Regression Analysis
Reproducibility of Results
Sensitivity and Specificity
Triticum
Wheat
Zea mays
Zearalenone
Zearalenone - analysis
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Summary:A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1800085