A 21.6 kDa tegumental protein of Clonorchis sinensis induces a Th1/Th2 mixed immune response in mice

Introduction Clonorchis sinensis is a major parasite affecting the Korea population. Despite the high infection rate and pathogenicity, very few studies have been conducted to investigate the immune responses against the proteins of C. sinensis. Methods In this study, in vitro immune response induce...

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Published in:Immunity, Inflammation and Disease Inflammation and Disease, 2018-12, Vol.6 (4), p.435-447
Main Authors: Chung, EunJoo, Kim, Yu Jung, Lee, Myoung‐Ro, Cho, Shin‐Hyeong, Ju, Jung‐Won
Format: Article
Language:English
Subjects:
Antigens
Clonorchis sinensis
Cytokines
dendritic cells
Disease control
Ethics
Experiments
Infections
Liver
Medical research
Original Research
Parasites
Prevention
Proteins
Studies
T lymphocytes
Tropical diseases
Vaccines
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Summary:Introduction Clonorchis sinensis is a major parasite affecting the Korea population. Despite the high infection rate and pathogenicity, very few studies have been conducted to investigate the immune responses against the proteins of C. sinensis. Methods In this study, in vitro immune response induced by a recombinant 21.6 kDa tegumental protein derived from C. sinensis (rCsTegu21.6) was confirmed in murine dendritic cells and T cells. For the in vivo analysis, each mouse was immunized three times. Total serum IgG and T cell cytokine production were determined by ELISA, while T cell proliferation was detected by a WST (Water‐Soluble Tetrazolium salt)‐1 assay. Results In vitro tests indicated that rCsTegu21.6 treatment increased the expression of surface molecules, such as CD40 (77%), CD80 (52%) and CD86 (46%), on murine dendritic cells and the secretion of cytokines (TNF‐α, IL‐6, IL‐1β, IL‐10, and IL‐12p70). Moreover, co‐culturing dendritic cells activated by rCsTegu21.6 with allogenic T cells induced T cell proliferation over time. rCsTegu21.6 also stimulated specific antibody production and cytokine secretion [IL‐2, IL‐4, and interferon (IFN)‐γ)] from T cells following immunization in vivo. Notably, rCsTegu21.6 predominantly induced IgG1 production and secretion of the Th2 cytokine IL‐4, regardless of the type of adjuvant used. Conclusion These results serve as a foundation for the development of tegumental protein‐based vaccines against C. sinensis. The liver fluke Clonorchis sinensis is a well‐known fish‐borne trematode that can survive in the bile ducts and cause various diseases. In this study, we characterized a 21.6 kDa tegumental protein and evaluated its immunological potential for further vaccine development.
ISSN:2050-4527
2050-4527
DOI:10.1002/iid3.235