Nezara viridula (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics

Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physi...

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Bibliographic Details
Published in:Scientific reports 2018-11, Vol.8 (1), p.17244-15, Article 17244
Main Authors: Lavore, Andrés, Perez-Gianmarco, Lucila, Esponda-Behrens, Natalia, Palacio, Victorio, Catalano, Maria Ines, Rivera-Pomar, Rolando, Ons, Sheila
Format: Article
Language:English
Subjects:
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Agricultural economics
Economic importance
Economics
Gene expression
Genomes
Hemiptera
Humanities and Social Sciences
Liquid chromatography
Mass spectrometry
Mass spectroscopy
multidisciplinary
Neuroendocrine system
Neuropeptides
Nezara viridula
Nucleotide sequence
Pentatomidae
Pests
Science
Science (multidisciplinary)
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Summary:Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physiological level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identified neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identified in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys , another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identified both group-specific features (which could be related to the different feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-35386-4