Advances in metabolic flux analysis toward genome-scale profiling of higher organisms

Methodological and technological advances have recently paved the way for metabolic flux profiling in higher organisms, like plants. However, in comparison with omics technologies, flux profiling has yet to provide comprehensive differential flux maps at a genome-scale and in different cell types, t...

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Bibliographic Details
Published in:Bioscience reports 2018-12, Vol.38 (6)
Main Authors: Basler, Georg, Fernie, Alisdair R, Nikoloski, Zoran
Format: Article
Language:English
Subjects:
Genome - genetics
Humans
Metabolic Flux Analysis - trends
Metabolic Networks and Pathways - genetics
Metabolomics - trends
Review
Systems Biology
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Summary:Methodological and technological advances have recently paved the way for metabolic flux profiling in higher organisms, like plants. However, in comparison with omics technologies, flux profiling has yet to provide comprehensive differential flux maps at a genome-scale and in different cell types, tissues, and organs. Here we highlight the recent advances in technologies to gather metabolic labeling patterns and flux profiling approaches. We provide an opinion of how recent local flux profiling approaches can be used in conjunction with the constraint-based modeling framework to arrive at genome-scale flux maps. In addition, we point at approaches which use metabolomics data without introduction of label to predict either non-steady state fluxes in a time-series experiment or flux changes in different experimental scenarios. The combination of these developments allows an experimentally feasible approach for flux-based large-scale systems biology studies.
ISSN:0144-8463
1573-4935
DOI:10.1042/BSR20170224