Response to chromate challenge by marine Staphylococcus sp. NIOMR8 evaluated by differential protein expression

Liquid Chromatography–Mass Spectrometry-Quadrupole Time of Flight (LC/MS QToF) protein profiling of marine-derived Staphyloccous gallinarum NIOMR8 was carried out to evaluate proteins conferring chromate (Cr 6+ ) resistance and possible metabolic pathways that were altered as a result. Expressional...

Full description

Saved in:
Bibliographic Details
Published in:3 Biotech 2018-12, Vol.8 (12), p.500-6, Article 500
Main Authors: Pereira, Elroy Joe, Damare, Samir, Furtado, Bliss, Ramaiah, Nagappa
Format: Article
Language:English
Subjects:
Agriculture
Amino acids
Biofilms
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Carbohydrates
Cell walls
Chemistry
Chemistry and Materials Science
Chromate
Chromates
Deoxyribonucleic acid
DNA
DNA repair
Functionals
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolic pathways
Metabolism
Nucleotides
Oxygen
Proteins
Quadrupoles
Repair
Short Reports
Stem Cells
Toxicity
Transcription
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Liquid Chromatography–Mass Spectrometry-Quadrupole Time of Flight (LC/MS QToF) protein profiling of marine-derived Staphyloccous gallinarum NIOMR8 was carried out to evaluate proteins conferring chromate (Cr 6+ ) resistance and possible metabolic pathways that were altered as a result. Expressional (up or down-regulation) responses to varying Cr 6+ (0, 50, 100, 150, and 200 µg mL − 1 ) concentrations varied, with as many as 346 proteins identified. Most number of proteins—their numbers in parentheses—were up-regulated when grown in medium with 50 µg mL − 1 (162) and, down-regulated in medium with 100 (281) or 200 µg mL − 1 Cr 6+ (280). Among these, eight proteins were commonly up-regulated, while 58 were commonly down-regulated across all conditions of Cr 6+ . Expression of protein moieties in metabolic pathways like translation (38), transcription (14), replication (18) and repair (4), metabolism of carbohydrates (26), amino acids (27), nucleotides (17), and membrane transport (21) was evidenced. Up-regulation patterns suggest that reduction of molecular oxygen (5), DNA repair (4) and peptide misfolding (7) were the potential protective mechanisms employed to counter Cr 6+ stress. Additionally, proteins associated with biofilm and cell wall biogenesis highlight their hypothetical involvement in toxicity tolerance. Results also indicate that at higher concentrations of Cr 6+ , down-regulation of functional proteins impedes normal cellular functions.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-018-1522-6