Legume Cytosolic and Plastid Acetyl-Coenzyme-A Carboxylase Genes Differ by Evolutionary Patterns and Selection Pressure Schemes Acting before and after Whole-Genome Duplications

Acetyl-coenzyme A carboxylase (ACCase, E.C.6.4.1.2) catalyzes acetyl-coenzyme A carboxylation to malonyl coenzyme A. Plants possess two distinct ACCases differing by cellular compartment and function. Plastid ACCase contributes to de novo fatty acid synthesis, whereas cytosolic enzyme to the synthes...

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Published in:Genes 2018-11, Vol.9 (11), p.563
Main Authors: Szczepaniak, Anna, Książkiewicz, Michał, Podkowiński, Jan, Czyż, Katarzyna B, Figlerowicz, Marek, Naganowska, Barbara
Format: Article
Language:English
Subjects:
Anthocyanins
Artificial chromosomes
Bacterial artificial chromosomes
Bayesian analysis
Carboxylation
Cloning
Coenzyme A
Enzymes
Evolution
Fatty acids
Flavonoids
Fluorescence in situ hybridization
Gene expression
Gene mapping
Genes
Genomes
Hybridization
Legumes
Phylogeny
Phytoalexins
Plastids
Positive selection
Proteins
Seeds
Synteny
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Summary:Acetyl-coenzyme A carboxylase (ACCase, E.C.6.4.1.2) catalyzes acetyl-coenzyme A carboxylation to malonyl coenzyme A. Plants possess two distinct ACCases differing by cellular compartment and function. Plastid ACCase contributes to de novo fatty acid synthesis, whereas cytosolic enzyme to the synthesis of very long chain fatty acids, phytoalexins, flavonoids, and anthocyanins. The narrow leafed lupin ( L.) represents legumes, a plant family which evolved by whole-genome duplications (WGDs). The study aimed on the contribution of these WGDs to the multiplication of ACCase genes and their further evolutionary patterns. The molecular approach involved bacterial artificial chromosome (BAC) library screening, fluorescent in situ hybridization, linkage mapping, and BAC sequencing. In silico analysis encompassed sequence annotation, comparative mapping, selection pressure calculation, phylogenetic inference, and gene expression profiling. Among sequenced legumes, the highest number of ACCase genes was identified in lupin and soybean. The most abundant plastid ACCase subunit genes were . ACCase genes in legumes evolved by WGDs, evidenced by shared synteny and Bayesian phylogenetic inference. Transcriptional activity of almost all copies was confirmed. Gene duplicates were conserved by strong purifying selection, however, positive selection occurred in ( ) and ( ) lineages, putatively predating the WGD event(s). Early duplicated and genes underwent transcriptional sub-functionalization.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes9110563