Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat

Extra-intestinal E. coli are emerging as a global threat due to their diffusion as opportunistic pathogens and, above all, to their wide set of antibiotic resistance determinants. There are still many gaps in our knowledge of their origin and spread pathways, although food animals have been adjudica...

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Bibliographic Details
Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-7
Main Authors: Fasciana, Teresa, Mascarella, Chiara, Oliveri, Giuseppa, Cardamone, Cinzia, Anna, Giammanco, Caruso, Giorgia, Capra, Giuseppina
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacteria
Chemotherapy
Drug resistance
Drug resistance in microorganisms
E coli
Farms
Food
Genes
Imipenem
Infections
Intestine
Laboratories
Meat
Multidrug resistance
Opportunist infection
Pathogens
Phylogenetics
Phylogeny
Proteins
Retail stores
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Stores
Strains (organisms)
Tetracycline
Tetracyclines
Veterinary medicine
Virulence
Virulence (Microbiology)
Virulence factors
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Summary:Extra-intestinal E. coli are emerging as a global threat due to their diffusion as opportunistic pathogens and, above all, to their wide set of antibiotic resistance determinants. There are still many gaps in our knowledge of their origin and spread pathways, although food animals have been adjudicated vehicles for passing mult-drug resistant bacteria to humans. This study analyzed 46 samples of meat purchased from retail stores in Palermo in order to obtain quinolone-resistant E. coli isolates. Strains were screened for their phylogenetic groups, ST131-associated single nucleotide polymorphisms (SNPs), and then typed by ERIC-PCR. Their set of virulence factors, namely, kpsMII, papA, sfaS, focG, iutA, papC, hlyD, and afa genes, were investigated and their fluoroquinolone-resistance determinants evaluated. The data obtained show a dramatically high prevalence of multidrug resistance patterns in the Palermo area, with 28% of the isolates having virulence factor genes typical of ExPEC strains. No B2 group or ST131 strains were detected. Moreover, 20% of our isolates showed positivity to all the plasmid-mediated quinolone resistance (PMQR) determinants, showing a potential to transfer these genes among other bacteria. Therefore, these data underline the possibility that food animals and, specifically, poultry in particular may be a significant source of resistant bacterial strains, posing a potential zoonotic risk.
ISSN:2314-6133
2314-6141
DOI:10.1155/2018/8714975