PSXII-21 CLA supplementation affects inflammatory and metabolic responses during an intramammary LPS challenge in early lactating dairy cows

• Published in: Journal of animal science 2018-12, Vol.96 (suppl_3), p.266-267
• Main Authors: Gross, J, Grossen-Rösti, L, Heritier, R, Troescher, A, Bruckmaier, R
• Format: Article
• Language: English
• Subjects: Abstracts
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/sky404.584

Abstract We investigated the effects of CLA in early lactating dairy cows exposed to an intramammary LPS challenge. Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c12 and 6.6 g of c9,t11 CLA isomer), CON: 56 g/d control fat). An intramammary LPS challenge (50 μg LPS, E. coli O26:B6) was performed in wk 4 p.p.. Blood and milk were sampled every 30 and 60 min. Plasma was analyzed for glucose, free fatty acids (FFA), beta-hydroxybutyrate (BHB), cortisol, insulin, and glucagon. In milk, SCC and activity of lactate dehydrogenase (LDH) were determined. Metabolic, clinical, and endocrine responses were evaluated using mixed models with time, group and the time × group interaction as fixed effects and the individual cow as repeated subject. Differences between CLA and CON groups were assessed by Bonferroni corrected t-tests at P