Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

Background. Mouse models of hypercholesterolaemia have been used to identify arterial proteins involved in atherosclerosis. As the liver is extremely sensitive to dyslipidemia, one might expect major changes in the abundance of liver proteins in these models even before atherosclerosis develops. Met...

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Bibliographic Details
Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-11
Main Authors: Kleffmann, Torsten, Legge, Michael, Jones, Gregory T., Porteous, Carolyn M., Rodger, Euan J., McCormick, Sally P. A.
Format: Article
Language:English
Subjects:
Analysis
Animal models
Animals
Antioxidants
Antioxidants - metabolism
Apolipoproteins
Arteriosclerosis
Atherosclerosis
Atherosclerosis - metabolism
Biology
Cell Line, Tumor
Cholesterol
Disease Models, Animal
Dyslipidemia
Dyslipidemias - metabolism
Efflux
Electrophoresis
Ethylenediaminetetraacetic acid
Exports
Female
Gel electrophoresis
Genetic engineering
Hep G2 Cells
Hepatocytes
Humans
Hypercholesterolemia
Hypercholesterolemia - metabolism
International trade
Lipid metabolism
Lipid Metabolism - physiology
Lipids
Lipoprotein A
Lipoprotein(a) - metabolism
Lipoproteins, LDL - metabolism
Liver
Liver - metabolism
Low density lipoprotein
Low density lipoprotein receptors
Low density lipoproteins
Mass spectrometry
Mass spectroscopy
Measurement methods
Metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Oxidative stress
Oxidative Stress - physiology
PARK7 protein
Phospholipids
Proteins
Proteins - metabolism
Proteomics - methods
Rodents
Scientific imaging
Studies
Superoxide dismutase
Thrombosis
Transgenic animals
Transgenic mice
Triglycerides
Two dimensional analysis
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Summary:Background. Mouse models of hypercholesterolaemia have been used to identify arterial proteins involved in atherosclerosis. As the liver is extremely sensitive to dyslipidemia, one might expect major changes in the abundance of liver proteins in these models even before atherosclerosis develops. Methods. Lipid levels were measured and a proteomic approach was used to quantify proteins in the livers of mice with an elevated low-density lipoprotein (LDL) and the presence of lipoprotein(a) [Lp(a)] but no atherosclerosis. Results. The livers of Lp(a) mice showed an increased triglyceride but reduced phospholipid and oxidised lipid content. Two-dimensional gel electrophoresis and mass spectrometry analysis identified 24 liver proteins with significantly increased abundance in Lp(a) mice (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2018/4963942