Holographic Characterization of Protein Aggregates

We demonstrate how holographic video microscopy can be used to detect, count, and characterize individual micrometer-scale protein aggregates as they flow down a microfluidic channel in their native buffer. Holographic characterization directly measures the radius and refractive index of subvisible...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2016-03, Vol.105 (3), p.1074-1085
Main Authors: Wang, Chen, Zhong, Xiao, Ruffner, David B., Stutt, Alexandra, Philips, Laura A., Ward, Michael D., Grier, David G.
Format: Article
Language:English
Subjects:
Animals
biopharmaceuticals characterization
Cattle
colloids
Dynamic Light Scattering - methods
Insulin - chemistry
light scattering
Microfluidics - methods
microparticles
microscopy
Microscopy, Video - methods
Pancreas - metabolism
particle size
Polyelectrolytes - chemistry
Protein Aggregates
protein aggregation
Proteins - chemistry
Refractometry
Serum Albumin, Bovine - chemistry
Silicones - chemistry
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Summary:We demonstrate how holographic video microscopy can be used to detect, count, and characterize individual micrometer-scale protein aggregates as they flow down a microfluidic channel in their native buffer. Holographic characterization directly measures the radius and refractive index of subvisible protein aggregates and offers insights into their morphologies. The measurement proceeds fast enough to build up population averages for time-resolved studies and lends itself to tracking trends in protein aggregation arising from changing environmental factors. Information on individual particle's refractive indexes can be used to differentiate protein aggregates from such contaminants as silicone droplets. These capabilities are demonstrated through measurements on samples of bovine pancreas insulin aggregated through centrifugation and of bovine serum albumin aggregated by complexation with a polyelectrolyte. Differentiation is demonstrated with samples that have been spiked with separately characterized silicone spheres. Holographic characterization measurements are compared with results obtained with microflow imaging and dynamic light scattering.
ISSN:0022-3549
1520-6017
DOI:10.1016/j.xphs.2015.12.018