DNA methylomes of bovine gametes and in vivo produced preimplantation embryos

• Published in: Biology of reproduction 2018-11, Vol.99 (5), p.949-959
• Main Authors: Jiang, Zongliang, Lin, Jianan, Dong, Hong, Zheng, Xinbao, Marjani, Sadie L, Duan, Jingyue, Ouyang, Zhengqing, Chen, Jingbo, Tian, Xiuchun (Cindy)
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Beef cattle; Biochemistry; Blastocyst - metabolism; bovine; Cattle; Deoxyribonucleic acid; DNA; DNA Methylation; DNA Transposable Elements; Embryonic development; Epigenetic inheritance; Epigenetics; Female; gametes; Gene expression; Genes; Genetic research; Genome; Genomes; Genomics; Germ Cells - metabolism; Male; Methylation; Oocytes - metabolism; Parenting; Povidone; Pregnancy; preimplantation embryos; Reproductive health; RRBS; Sequence Analysis, DNA; Sperm; Spermatozoa - chemistry; Sulfites; Terminal Repeat Sequences
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/ioy138

DNA methylation is an important epigenetic modification that undergoes dynamic changes in mammalian embryogenesis, during which both parental genomes are reprogrammed. Despite the many immunostaining studies that have assessed global methylation, the gene-specific DNA methylation patterns in bovine preimplantation embryos are unknown. Using reduced representation bisulfite sequencing, we determined genome-scale DNA methylation of bovine sperm and individual in vivo developed oocytes and preimplantation embryos. We show that (1) the major wave of genome-wide demethylation was completed by the 8-cell stage; (2) promoter methylation was significantly and inversely correlated with gene expression at the 8-cell and blastocyst stages; (3) sperm and oocytes have numerous differentially methylated regions (DMRs)—DMRs specific for sperm were strongly enriched in long terminal repeats and rapidly lost methylation in embryos; while the oocyte-specific DMRs were more frequently localized in exons and CpG islands (CGIs) and demethylated gradually across cleavage stages; (4) DMRs were also found between in vivo and in vitro matured oocytes; and (5) differential methylation between bovine gametes was confirmed in some but not all known imprinted genes. Our data provide insights into the complex epigenetic reprogramming of bovine early embryos, which serve as an important model for human preimplantation development. Summary Sentence Genome-wide gene-specific DNA methylation in bovine sperm, oocytes and preimplantation embryos.