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Sequencing the Dermatan Sulfate Chain of Decorin

Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis. One of the most structurally complex clas...

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Published in:Journal of the American Chemical Society 2017-11, Vol.139 (46), p.16986-16995
Main Authors: Yu, Yanlei, Duan, Jiana, Leach, Franklin E, Toida, Toshihiko, Higashi, Kyohei, Zhang, Hong, Zhang, Fuming, Amster, I. Jonathan, Linhardt, Robert J
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cited_by cdi_FETCH-LOGICAL-a483t-ecffb33d711f6618cded81a17ef6988b29faaa522f0219d1e008215bfc95de173
cites cdi_FETCH-LOGICAL-a483t-ecffb33d711f6618cded81a17ef6988b29faaa522f0219d1e008215bfc95de173
container_end_page 16995
container_issue 46
container_start_page 16986
container_title Journal of the American Chemical Society
container_volume 139
creator Yu, Yanlei
Duan, Jiana
Leach, Franklin E
Toida, Toshihiko
Higashi, Kyohei
Zhang, Hong
Zhang, Fuming
Amster, I. Jonathan
Linhardt, Robert J
description Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis. One of the most structurally complex classes of glycoconjugates is the proteoglycans (PGs) and their glycosaminoglycan (GAG) side chains. Previously, our laboratory solved the structure of the chondroitin sulfate chain of the bikunin PG. The current study examines the much more complex structure of the dermatan sulfate GAG chain of decorin PG. By utilizing sophisticated separation methods followed by compositional analysis, domain mapping, and tandem mass spectrometry coupled with analysis by a modified genetic algorithm approach, the structural motif for the decorin dermatan sulfate chain was determined. This represents the second example of a GAG with a prominent structural motif, suggesting that the structural variability of this class of glycoconjugates is somewhat simpler than had been expected.
doi_str_mv 10.1021/jacs.7b10164
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Algorithms
Animals
Decorin - chemistry
Decorin - isolation & purification
Dermatan Sulfate - chemistry
Dermatan Sulfate - isolation & purification
Swine
title Sequencing the Dermatan Sulfate Chain of Decorin
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