Inter-Kingdom beach warfare: Microbial chemical communication activates natural chemical defences

An inter-kingdom beach warfare between a Streptomyces sp. and Aspergillus sp. co-isolated from shallow water beach sand, collected off Heron Island, Queensland, Australia, saw the bacteriostatic Aspergillus metabolite cyclo -( l -Phe- trans -4-hydroxy- l -Pro) ( 3 ) stimulate the Streptomyces to pro...

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Bibliographic Details
Published in:The ISME Journal 2019-01, Vol.13 (1), p.147-158
Main Authors: Khalil, Zeinab G., Cruz-Morales, Pablo, Licona-Cassani, Cuauhtemoc, Marcellin, Esteban, Capon, Robert J.
Format: Article
Language:English
Subjects:
631/158/855
704/158/855
Aspergillus
Aspergillus - genetics
Aspergillus - physiology
Beaches
Biomedical and Life Sciences
Chemical communication
Chemical defense
Ecology
Environmental Microbiology
Evolutionary Biology
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Fungal
Life Sciences
Metabolites
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microorganisms
Nitric oxide
Nitric Oxide Synthase
Organic chemistry
Queensland
Shallow water
Soil
Soil Microbiology
Streptomyces
Streptomyces - genetics
Streptomyces - physiology
Transcription activation
Warfare
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Summary:An inter-kingdom beach warfare between a Streptomyces sp. and Aspergillus sp. co-isolated from shallow water beach sand, collected off Heron Island, Queensland, Australia, saw the bacteriostatic Aspergillus metabolite cyclo -( l -Phe- trans -4-hydroxy- l -Pro) ( 3 ) stimulate the Streptomyces to produce nitric oxide (NO), which in turn mediated transcriptional activation of a silent biosynthetic gene cluster (BGC) for fungistatic heronapyrrole B ( 1 ). Structure activity relationship studies, coupled with the use of NO synthase inhibitors, donors and scavangers, and both genomic and transcriptomic analyses, confirmed the extraordinary chemical cue specificity of 3 , and its NO-mediated mechanism of transcriptional action. Our findings reveal the importance of inter-kingdom (fungal-bacterial) chemical communication in the regulation of silent BGCs coding for chemical defenses. We propose that the detection and characterisation of NO mediated transcriptional activation (NOMETA) of silent chemical defences in the environment, may inspire broader application in the field of microbial biodiscovery.
ISSN:1751-7362
1751-7370
DOI:10.1038/s41396-018-0265-z