In vitro conservation of embryogenic cultures of date palm using osmotic mediated growth agents

This study was carried out to investigate the effect of mannitol, sorbitol and sucrose as osmotic agents on in vitro conservation of embryogenic cultures of date palm (Phoenix dactylifera, L.) Bartamoda and Sakkoty cultivars. Embryogenic cultures was obtained using MS medium supplemented with 10mg/l...

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Bibliographic Details
Published in:Journal of Genetic Engineering and Biotechnology 2016-12, Vol.14 (2), p.363-370
Main Authors: El-Bahr, M.K., Abd EL-Hamid, A., Matter, M.A., Shaltout, A., Bekheet, S.A., El-Ashry, A.A.
Format: Article
Language:English
Subjects:
2,4-D
cultivars
Date palm
genetic engineering
genetic stability
genetic variation
In vitro preservation
Mannitol
Phoenix dactylifera
random amplified polymorphic DNA technique
Sorbitol
storage time
Sucrose
V : Plant Biotechnology
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Summary:This study was carried out to investigate the effect of mannitol, sorbitol and sucrose as osmotic agents on in vitro conservation of embryogenic cultures of date palm (Phoenix dactylifera, L.) Bartamoda and Sakkoty cultivars. Embryogenic cultures was obtained using MS medium supplemented with 10mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 3mg/l isopentenyl adenine (2iP). Among the three types of osmotic substances used for slow growth conservation, sucrose at all concentrations gave the highest percentage of survival with Sakkoty cultivar. However, addition of 40g/l or 60g/l mannitol and 20g/l sorbitol showed the highest percentage of survival percentage with Bartamoda cultivar. The different sucrose concentrations caused higher numbers of germinated embryos of the two cultivars compared with mannitol or sorbitol. Also, the number of germinated embryos was increased with increasing the storage periods till the ninth month. Genetic stability was determined using random amplified polymorphic DNA (RAPD) analysis. There were no clear genetic differences between the two osmotic agents used for preservation. The preserved cultures of Sakkoty cultivar gave the high percent of similarity while Bartamoda cultivar gave low percent of similarity. From the obtained results we can recommend using 40g/l mannitol or 20g/l sorbitol for in vitro preservation of Bartamoda cultivar of date palm and 20g/l of sucrose for Sakkoty cultivar.
ISSN:1687-157X
2090-5920
DOI:10.1016/j.jgeb.2016.08.004