Methotrexate-induced apoptosis in human ovarian adenocarcinoma SKOV-3 cells via ROS-mediated bax/bcl-2-cyt-c release cascading

The communication between a substance and a cell may depend on whether the cell is normal or pathological. The disease cells and drug interaction may occasionally overcome beneficial action of the drug; subsequently, it is important to investigate the effect of the drug in both the normal and target...

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Bibliographic Details
Published in:OncoTargets and therapy 2019-01, Vol.12, p.21-30
Main Authors: AlBasher, Gadah, AlKahtane, Abdullah A, Alarifi, Saud, Ali, Daoud, Alessia, Mohammed S, Almeer, Rafa S, Abdel-Daim, Mohamed M, Al-Sultan, Nouf K, Al-Qahtani, Ahmed A, Ali, Huma, Alkahtani, Saad
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Cancer
Cancer therapies
Cell cycle
Cell growth
Chemotherapy
Cytochrome
Deoxyribonucleic acid
DNA
DNA damage
Ethylenediaminetetraacetic acid
Gene expression
Lung cancer
Medical prognosis
Metabolism
Methotrexate
Microscopy
Mitochondria
Morphology
Original Research
Ovarian cancer
Permeability
Photographic industry
Prevention
Scientific equipment and supplies industry
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Summary:The communication between a substance and a cell may depend on whether the cell is normal or pathological. The disease cells and drug interaction may occasionally overcome beneficial action of the drug; subsequently, it is important to investigate the effect of the drug in both the normal and target cells. This study aimed to evaluate the methotrexate (MTX) antiproliferative effect and explore the mechanistic approach to investigate the cell death index in SKOV-3 ovarian cells during treatment with MTX. In vitro studies of SKOV-3 cells were examined by tetrazolium assay after exposure to various concentrations of MTX. Moreover, reactive oxygen species (ROS) generation, mitochondrial membrane potential, DNA damage, and AO/EtBr staining morphological analysis of necrotic/apoptotic cells were detected; cellular impairment in mitochondria and DNA was confirmed by JC-1 mitotracker/DAPI, respectively, and cell death pathway markers; bax/bcl-2 were analyzed. A dose-dependent antiproliferative effect of MTX treatment was observed in SKOV-3 cells; the prominent inhibitory concentration was 40 µM of MTX (
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S178510