Interactions of Functionalized Multi-Wall Carbon Nanotubes with Giant Phospholipid Vesicles as Model Cellular Membrane System

• Published in: Scientific reports 2018-12, Vol.8 (1), p.17998-11, Article 17998
• Main Authors: Pérez-Luna, Verónica, Moreno-Aguilar, Carlos, Arauz-Lara, José Luis, Aranda-Espinoza, Said, Quintana, Mildred
• Format: Article
• Language: English
• Subjects: 639/301/923/966; 639/925/357/73; Carbon; Cell Membrane - pathology; Cell membranes; Coated Materials, Biocompatible - chemical synthesis; Coated Materials, Biocompatible - chemistry; Confocal microscopy; Drug development; Gene transfer; Humanities and Social Sciences; Hydrophobic and Hydrophilic Interactions; Hydrophobic surfaces; Hydrophobicity; Lipid membranes; Materials Testing; Mathematical models; Mechanical Phenomena; Mechanical properties; Membrane vesicles; Membranes; Membranes, Artificial; Models, Biological; multidisciplinary; Nanotechnology; Nanotubes; Nanotubes, Carbon - chemistry; Phospholipids; Phospholipids - chemistry; Science; Science (multidisciplinary); Statistical analysis; Surface Properties
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-36531-9

Carbon Nanotubes (CNTs) are considered alternative materials for the design of advanced drug and gene delivery vectors. However, the mechanism responsible for the cellular membrane intake of CNTs is not well understood. In the present study, we show how multi-walled carbon nanotubes (MWCNTs) owning different surface properties, interact with giant unilamellar vesicles (GUVs), a simple model system for cellular membranes. In particular, we want to address the hydrophilic/hydrophobic interactions between MWCNTs and lipid membranes and the subsequent mechanical properties changes of the systems. In order to elucidate this interaction, we made the following chemical modifications on MWCNTs: oxidized MWCNTs (ox-MWCNTs) displaying reduced hydrophobic surface character, pristine MWCNTs (p-MWCNTs), and alkyl functionalized MWCNTs (alk-MWCNTs) exhibiting enhanced hydrophobic surface properties, were put in contact with GUVs and observed by confocal microscopy. Our observations revealed that the interaction between the CNTs and GUVs depends on the type of chemical functionalization: ox-MWCNTs remain at the membrane interacting with the polar head of the phospholipids, p-MWCNTs internalize GUVs spontaneously, and alk-MWCNTs persist inside the membrane. The mechanical properties of MWCNTs@GUVs systems were measured using the electrodeformation method, which shows an increased bending stiffness ( κ ) of the GUVs as MWCNTs concentration increases. High concentrations of p-MWCNTs and alk-MWCNTs induced vesicle adhesion; p-MWCNTs produced a considerable reduction in the average size of the GUVs, while alk-MWCNTs form complex stable structures inside the membrane. The statistical analyses of the experimental results are compared with available computer simulations. The picture emerging from our results is that the interaction between GUVs and MWCNTs is due mainly to hydrophobicity.