Genome of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae), a worldwide parasite of social bee colonies, provides insights into detoxification and herbivory

• Published in: Gigascience 2018-12, Vol.7 (12)
• Main Authors: Evans, Jay D, McKenna, Duane, Scully, Erin, Cook, Steven C, Dainat, Benjamin, Egekwu, Noble, Grubbs, Nathaniel, Lopez, Dawn, Lorenzen, Marcé D, Reyna, Steven M, Rinkevich, Frank D, Neumann, Peter, Huang, Qiang
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - classification; Acetylcholinesterase - genetics; Acetylcholinesterase - metabolism; Aethina tumida; Animals; Apis mellifera; ATP-Binding Cassette Transporters - classification; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Beehives; Bees; Bees - parasitology; Beetles; Coleoptera; Coleoptera - classification; Coleoptera - genetics; Colonies; Detoxification; digestion; Gene families; genes; Genetic Variation; Genome; genome assembly; Genomes; Genomics; Glycoside Hydrolases - classification; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; herbivores; Herbivory; Homology; honey; honey bees; Insect Proteins - classification; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticides; Insecticides - metabolism; Insects; Invasiveness; metabolism; nests; Nitidulidae; parasites; Phylogeny; Pollinators; Receptors; Receptors, Cell Surface - classification; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Species; taste; Taste receptors; Voltage-Gated Sodium Channels - classification; Voltage-Gated Sodium Channels - genetics
• ISSN: 2047-217X; 2047-217X
• DOI: 10.1093/gigascience/giy138

The small hive beetle (Aethina tumida; ATUMI) is an invasive parasite of bee colonies. ATUMI feeds on both fruits and bee nest products, facilitating its spread and increasing its impact on honey bees and other pollinators. We have sequenced and annotated the ATUMI genome, providing the first genomic resources for this species and for the Nitidulidae, a beetle family that is closely related to the extraordinarily species-rich clade of beetles known as the Phytophaga. ATUMI thus provides a contrasting view as a neighbor for one of the most successful known animal groups. We present a robust genome assembly and a gene set possessing 97.5% of the core proteins known from the holometabolous insects. The ATUMI genome encodes fewer enzymes for plant digestion than the genomes of wood-feeding beetles but nonetheless shows signs of broad metabolic plasticity. Gustatory receptors are few in number compared to other beetles, especially receptors with known sensitivity (in other beetles) to bitter substances. In contrast, several gene families implicated in detoxification of insecticides and adaptation to diverse dietary resources show increased copy numbers. The presence and diversity of homologs involved in detoxification differ substantially from the bee hosts of ATUMI. Our results provide new insights into the genomic basis for local adaption and invasiveness in ATUMI and a blueprint for control strategies that target this pest without harming their honey bee hosts. A minimal set of gustatory receptors is consistent with the observation that, once a host colony is invaded, food resources are predictable. Unique detoxification pathways and pathway members can help identify which treatments might control this species even in the presence of honey bees, which are notoriously sensitive to pesticides.