Douglas-Fir (Pseudotsuga menziesii (Mirb.) Franco) Transcriptome Profile Changes Induced by Diesel Emissions Generated with CeO2 Nanoparticle Fuel Borne Catalyst

It is important to understand molecular effects on plants exposed to compounds released from use of products containing engineered nanomaterials. Here, we present mRNA sequencing data on transcriptome impacts to Douglas-fir following 2 weeks of sublethal exposure to 30:1 diluted airborne emissions r...

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Bibliographic Details
Published in:Environmental science & technology 2018-09, Vol.52 (17), p.10067-10077
Main Authors: Reichman, Jay R, Rygiewicz, Paul T, Johnson, Mark G, Bollman, Michael A, Smith, Bonnie M, Krantz, Q. Todd, King, Charly J, Kovalcik, Kasey D, Andersen, Christian P
Format: Article
Language:English
Subjects:
Background levels
Baseline studies
Catalysis
Catalysts
Cerium oxides
Diesel
Diesel engines
Diesel fuels
Emissions
Enrichment
Environmental impact
Exhaust gases
Exposure
Gene expression
Gene sequencing
Nanomaterials
Nanoparticles
Nanotechnology
Pine needles
Plant physiology
Proteins
Pseudotsuga menziesii
Ribonucleic acid
RNA
Seedlings
Water pollution effects
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Summary:It is important to understand molecular effects on plants exposed to compounds released from use of products containing engineered nanomaterials. Here, we present mRNA sequencing data on transcriptome impacts to Douglas-fir following 2 weeks of sublethal exposure to 30:1 diluted airborne emissions released from combustion of diesel fuel containing engineered CeO2 nanoparticle catalysts (DECe). Our hypothesis was that chamber exposure to DECe would induce distinct transcriptome changes in seedling needles compared with responses to conventional diesel exhaust (DE) or filtered DECe Gas Phase. Significantly increased uptake/binding of Ce in needles of DECe treated seedlings was 2.7X above background levels and was associated with altered gene expression patterns. All 225 Blast2GO gene ontologies (GOs) enriched by up-regulated DECe transcripts were nested within GOs for DE, however, 29 of 31 enriched GOs for down-regulated DECe transcripts were unique. MapMan analysis also identified three pathways enriched with DECe down-regulated transcripts. There was prominent representation of genes with attenuated expression in transferase, transporter, RNA regulation and protein degradation GOs and pathways. CeO2 nanoparticle additive decreased and shifted molecular impact of diesel emissions. Wide-spread use of such products and chronic environmental exposure to DECe may adversely affect plant physiology and development.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.8b02169