Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells

Hybrid approaches combining gene- and cell-based therapies to make biological pacemakers are a promising therapeutic avenue for bradyarrhythmia. The present study aimed to direct adipose tissue-derived stem cells (ADSCs) to differentiate specifically into cardiac pacemaker cells by overexpressing a...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2019-02, Vol.43 (2), p.879-889
Main Authors: Zhang, Jian, Yang, Mei, Yang, An-Kang, Wang, Xi, Tang, Yan-Hong, Zhao, Qing-Yan, Wang, Teng, Chen, Yu-Ting, Huang, Cong-Xin
Format: Article
Language:English
Subjects:
Binding proteins
Body fat
Cardiac arrhythmia
Cardiac pacing
Cell differentiation
Gene expression
Genes
Genetic aspects
Genetic engineering
Health aspects
Heart
Infections
Insulin
Laboratory animals
Proteins
Sinuses
Stem cells
Studies
Transcription factors
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hybrid approaches combining gene- and cell-based therapies to make biological pacemakers are a promising therapeutic avenue for bradyarrhythmia. The present study aimed to direct adipose tissue-derived stem cells (ADSCs) to differentiate specifically into cardiac pacemaker cells by overexpressing a single transcription factor, insulin gene enhancer binding protein 1 (ISL-1). In the present study, the ADSCs were transfected with ISL‑1 or mCherry fluorescent protein lentiviral vectors and co-cultured with neonatal rat ventricular cardiomyocytes (NRVMs) in vitro for 5-7 days. The feasibility of regulating the differentiation of ADSCs into pacemaker-like cells by overexpressing ISL-1 was evaluated by observation of cell morphology and beating rate, reverse transcription-quantitative polymerase chain reaction analysis, western blotting, immunofluorescence and analysis of electrophysiological activity. In conclusion, these data indicated that the overexpression of ISL-1 in ADSCs may enhance the pacemaker phenotype and automaticity in vitro, features which were significantly increased following co‑culture induction.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2018.4002