Silver nanopaticles synthesized using the seed extract of Trigonella foenum-graecum L. and their antimicrobial mechanism and anticancer properties

• Published in: Saudi journal of biological sciences 2019-01, Vol.26 (1), p.148-154
• Main Authors: Varghese, Rakesh, Almalki, Mohammed A., Ilavenil, Soundharrajan, Rebecca, Jeyanthi, Choi, Ki Choon
• Format: Article
• Language: English
• Subjects: AgNPs; Anticancer activity; Antimicrobial activity; Antimicrobial mechanism; FTIR; Original; Trigonella foenum-graecum L; الأدوية المضادة للأورام; التخليق الحيوي; الجسيمات النانوية; الحلبة; السرطان; العلاج المناعي; الفضة; المستخلصات النباتية
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2017.07.001

Synthesis of silver nanoparticles (AgNPs) through biological route plays an important role in their applications in the medical field, especially in the prevention of disease causing microbial pathogens and arresting the propagation of cancer cells. The stable, green synthesis of AgNPs is very much welcomed in the medical field because of their low toxicity. Therefore, the demands of AgNPs synthesised biologically is on the rise. The present study aimed to investigate the antimicrobial mechanisms and anticancer properties of the AgNPs synthesized using the seed extract of Trigonella foenum-graecum L. The AgNPs were characterized by UV–vis, SEM, XRD, FTIR and EDAX analysis. The minimum inhibitory concentrations (MIC) of the AgNPs were determined by the broth micro dilution method. The formation of brownish red color indicated the formation NPs with the absorption maximum at 420nm. The average size was found to be 33.93nm and sphere shaped. The FTIR spectrum revealed the absorption bands at 3340cm−1 and 1635cm−1 indicated the presence of OH or COOH and amide group stretching in the AgNPs. The X-ray diffraction report confirmed the presence of strong peak values of 2θ within the angle of 37.1°. The lowest MIC of the AgNPs against Staphylococcus aureus was 62.5μgmL−1. MIC values against Escherichia coli and Klebsiella pneumonia, were 125 and 250μgmL−1 respectively. The MIC of the AgNPs against Aspergillus flavus, Trichophyton rubrum and Trichoderma viridiae were each 250μgmL−1, respectively. The extracellular protein concentration, levels of lactate dehydrogenase and alkaline phosphtase enzyme in the AgNPs treated bacterial pathogens demonstrated greater antimicrobial mechanism. Additionally, the AgNPs exhibited significant anticancer activity against the MCF7 and Vero cell lines. The synthesized AgNPs could be further evaluated in large scale as a botanical antimicrobial agent.