Overexpression of rice aquaporin OsPIP1;2 improves yield by enhancing mesophyll CO2 conductance and phloem sucrose transport

OsPIP1;2 overexpression enhances rice growth and grain yield by facilitating leaf CO2 diffusion, which increases the net CO2 assimilation rate and phloem sucrose transport. Abstract Aquaporins are involved in CO2 transport from the leaf intercellular air space to the chloroplast, which contributes t...

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Published in:Journal of experimental botany 2019-01, Vol.70 (2), p.671-681
Main Authors: Xu, Feiyun, Wang, Ke, Yuan, Wei, Xu, Weifeng, Liu, Shuang, Kronzucker, Herbert J, Chen, Guanglei, Miao, Rui, Zhang, Maoxing, Ding, Ming, Xiao, Liang, Kai, Lei, Zhang, Jianhua, Zhu, Yiyong
Format: Article
Language:English
Subjects:
Aquaporins - metabolism
Biomass
Carbon Dioxide - metabolism
Edible Grain - growth & development
Mesophyll Cells - metabolism
Oryza - growth & development
Oryza - metabolism
Phloem - metabolism
Photosynthesis
Research Papers
Sucrose - metabolism
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Summary:OsPIP1;2 overexpression enhances rice growth and grain yield by facilitating leaf CO2 diffusion, which increases the net CO2 assimilation rate and phloem sucrose transport. Abstract Aquaporins are involved in CO2 transport from the leaf intercellular air space to the chloroplast, which contributes to CO2 assimilation. However, the mechanism of CO2 transport by rice (Oryza sativa L.) aquaporins is unknown. Here, we investigated the function of the aquaporin OsPIP1;2 in CO2 diffusion-associated photosynthesis and phloem sucrose transport. Moreover, the grain yield of rice lines overexpressing OsPIP1;2 was determined. OsPIP1;2 was localized to the plasma membrane and the relative expression of OsPIP1;2 was approximately 5-fold higher in leaves in the presence of an elevated CO2 concentration. Overexpression of OsPIP1;2 increased mesophyll conductance by approximately 150% compared with wild-type (WT) rice. The OsPIP1;2-overexpressing lines had higher biomass than the WT, possibly due to increased phloem sucrose transport. In addition, the grain yield of OsPIP1;2-overexpressing lines was approximately 25% higher than that of the WT in three-season field experiments, due to the increased numbers of effective tillers and spikelets per panicle. Our results suggest that OsPIP1;2 modulates rice growth and grain yield by facilitating leaf CO2 diffusion, which increases both the net CO2 assimilation rate and sucrose transport.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/ery386