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Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme
In this study, novel, hollow superparamagnetic copper ferrite (CuFe 2 O 4 ) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscop...
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| Published in: | Indian journal of microbiology 2019-03, Vol.59 (1), p.105-108 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c470t-b04571344decc2ae8d39e752455e5df759bdd00f0dc264f26c330eb87b526bfd3 |
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| cites | cdi_FETCH-LOGICAL-c470t-b04571344decc2ae8d39e752455e5df759bdd00f0dc264f26c330eb87b526bfd3 |
| container_end_page | 108 |
| container_issue | 1 |
| container_start_page | 105 |
| container_title | Indian journal of microbiology |
| container_volume | 59 |
| creator | Otari, Sachin V. Patel, Sanjay K. S. Kim, Sang-Yong Haw, Jung Rim Kalia, Vipin C. Kim, In-Won Lee, Jung-Kul |
| description | In this study, novel, hollow superparamagnetic copper ferrite (CuFe
2
O
4
) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of
Thermomyces lanuginosus
lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g
−1
of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use. |
| doi_str_mv | 10.1007/s12088-018-0768-3 |
| format | article |
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2
O
4
) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of
Thermomyces lanuginosus
lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g
−1
of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use.</description><identifier>ISSN: 0046-8991</identifier><identifier>EISSN: 0973-7715</identifier><identifier>DOI: 10.1007/s12088-018-0768-3</identifier><identifier>PMID: 30728638</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Biological materials ; Biomedical and Life Sciences ; Confocal microscopy ; Copper ; Copper ferrite ; Enzymes ; Field emission microscopy ; Fourier transforms ; Immobilization ; Life Sciences ; Lipase ; Low temperature ; Medical Microbiology ; Microbiology ; Microscopy ; Morphology ; Nanoparticles ; Scanning electron microscopy ; Scanning microscopy ; Shelf life ; Short Communications ; Silicon ; Spectroscopy ; Transmission electron microscopy</subject><ispartof>Indian journal of microbiology, 2019-03, Vol.59 (1), p.105-108</ispartof><rights>Association of Microbiologists of India 2018</rights><rights>Indian Journal of Microbiology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-b04571344decc2ae8d39e752455e5df759bdd00f0dc264f26c330eb87b526bfd3</citedby><cites>FETCH-LOGICAL-c470t-b04571344decc2ae8d39e752455e5df759bdd00f0dc264f26c330eb87b526bfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328406/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328406/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30728638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Otari, Sachin V.</creatorcontrib><creatorcontrib>Patel, Sanjay K. S.</creatorcontrib><creatorcontrib>Kim, Sang-Yong</creatorcontrib><creatorcontrib>Haw, Jung Rim</creatorcontrib><creatorcontrib>Kalia, Vipin C.</creatorcontrib><creatorcontrib>Kim, In-Won</creatorcontrib><creatorcontrib>Lee, Jung-Kul</creatorcontrib><title>Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme</title><title>Indian journal of microbiology</title><addtitle>Indian J Microbiol</addtitle><addtitle>Indian J Microbiol</addtitle><description>In this study, novel, hollow superparamagnetic copper ferrite (CuFe
2
O
4
) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of
Thermomyces lanuginosus
lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g
−1
of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use.</description><subject>Biological materials</subject><subject>Biomedical and Life Sciences</subject><subject>Confocal microscopy</subject><subject>Copper</subject><subject>Copper ferrite</subject><subject>Enzymes</subject><subject>Field emission microscopy</subject><subject>Fourier transforms</subject><subject>Immobilization</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Low temperature</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Scanning electron microscopy</subject><subject>Scanning microscopy</subject><subject>Shelf life</subject><subject>Short Communications</subject><subject>Silicon</subject><subject>Spectroscopy</subject><subject>Transmission electron microscopy</subject><issn>0046-8991</issn><issn>0973-7715</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU1rFTEUhoMotlZ_gBsZcONm9OQ72Qhy6bWFqhtdh0zmzG3KzGRM5hbaX2_Kra0KLkIOnOe8yeEh5DWF9xRAfyiUgTEt0Hq0Mi1_Qo7Bat5qTeXTWoNQrbGWHpEXpVwBSGWVfE6OOGhmFDfHZLtJy4K52WLOccXmi9_NuMbQfPVzWnyu5YilGVJu1ktszqcpdXGMt36NaW7S0JzOtzcTviTPBj8WfHV_n5Af29Pvm7P24tvn882nizYIDWvbgZCaciF6DIF5ND23qCUTUqLsBy1t1_cAA_SBKTEwFTgH7IzuJFPd0PMT8vGQu-y7CfuA85r96JYcJ59vXPLR_d2Z46XbpWunODMCVA14dx-Q0889ltVNsQQcRz9j2hfHGLOgrdGiom__Qa_SPs91Pccot1xzTm2l6IEKOZWScXj4DAV3Z8kdLLlqyd1ZcrzOvPlzi4eJ31oqwA5Aqa15h_nx6f-n_gIXmZ3I</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Otari, Sachin V.</creator><creator>Patel, Sanjay K. S.</creator><creator>Kim, Sang-Yong</creator><creator>Haw, Jung Rim</creator><creator>Kalia, Vipin C.</creator><creator>Kim, In-Won</creator><creator>Lee, Jung-Kul</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190301</creationdate><title>Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme</title><author>Otari, Sachin V. ; Patel, Sanjay K. S. ; Kim, Sang-Yong ; Haw, Jung Rim ; Kalia, Vipin C. ; Kim, In-Won ; Lee, Jung-Kul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-b04571344decc2ae8d39e752455e5df759bdd00f0dc264f26c330eb87b526bfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biological materials</topic><topic>Biomedical and Life Sciences</topic><topic>Confocal microscopy</topic><topic>Copper</topic><topic>Copper ferrite</topic><topic>Enzymes</topic><topic>Field emission microscopy</topic><topic>Fourier transforms</topic><topic>Immobilization</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Low temperature</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Scanning electron microscopy</topic><topic>Scanning microscopy</topic><topic>Shelf life</topic><topic>Short Communications</topic><topic>Silicon</topic><topic>Spectroscopy</topic><topic>Transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Otari, Sachin V.</creatorcontrib><creatorcontrib>Patel, Sanjay K. S.</creatorcontrib><creatorcontrib>Kim, Sang-Yong</creatorcontrib><creatorcontrib>Haw, Jung Rim</creatorcontrib><creatorcontrib>Kalia, Vipin C.</creatorcontrib><creatorcontrib>Kim, In-Won</creatorcontrib><creatorcontrib>Lee, Jung-Kul</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Otari, Sachin V.</au><au>Patel, Sanjay K. S.</au><au>Kim, Sang-Yong</au><au>Haw, Jung Rim</au><au>Kalia, Vipin C.</au><au>Kim, In-Won</au><au>Lee, Jung-Kul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme</atitle><jtitle>Indian journal of microbiology</jtitle><stitle>Indian J Microbiol</stitle><addtitle>Indian J Microbiol</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>59</volume><issue>1</issue><spage>105</spage><epage>108</epage><pages>105-108</pages><issn>0046-8991</issn><eissn>0973-7715</eissn><abstract>In this study, novel, hollow superparamagnetic copper ferrite (CuFe
2
O
4
) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of
Thermomyces lanuginosus
lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g
−1
of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>30728638</pmid><doi>10.1007/s12088-018-0768-3</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Indian journal of microbiology, 2019-03, Vol.59 (1), p.105-108 |
| issn | 0046-8991 0973-7715 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6328406 |
| source | Springer Link; PubMed Central |
| subjects | Biological materials Biomedical and Life Sciences Confocal microscopy Copper Copper ferrite Enzymes Field emission microscopy Fourier transforms Immobilization Life Sciences Lipase Low temperature Medical Microbiology Microbiology Microscopy Morphology Nanoparticles Scanning electron microscopy Scanning microscopy Shelf life Short Communications Silicon Spectroscopy Transmission electron microscopy |
| title | Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme |
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