Bioassay for monitoring the anti-aging effect of cord blood treatment

Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed an impressive potential to slow age-associated degradation of neuronal and cognitive functions. Translating such findings to clinical realities, however, requires effective ways for assessing treatm...

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Bibliographic Details
Published in:Theranostics 2019, Vol.9 (1), p.1-10
Main Authors: Bae, Sang-Hun, Jo, Ala, Park, Jae Hyun, Lim, Chul-Woo, Choi, Yuri, Oh, Juhyun, Park, Ji-Min, Kong, TaeHo, Weissleder, Ralph, Lee, Hakho, Moon, Jisook
Format: Article
Language:English
Subjects:
Aging
Animals
Arachidonic Acid - blood
Biosensing Techniques - methods
Blood Transfusion
Drug Monitoring - methods
Electrochemical Techniques - methods
Fetal Blood
Longitudinal Studies
Magnetics - methods
Metabolomics - methods
Mice
Models, Animal
Plasma - chemistry
Research Paper
Treatment Outcome
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Summary:Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed an impressive potential to slow age-associated degradation of neuronal and cognitive functions. Translating such findings to clinical realities, however, requires effective ways for assessing treatment efficacy; ideal methods should be minimally invasive, amenable for serial assays, cost-effective, and quantitative. We developed a new biosensor approach to monitor anti-aging therapy. We advanced two key sensor components: i) a blood-borne metabolite was identified as a surrogate aging-marker; and ii) a compact and cost-effective assay system was developed for on-site applications. We treated aged mice either with human umbilical cord plasma or saline; unbiased metabolite profiling on mouse plasma revealed arachidonic acid (AA) as a potent indicator associated with anti-aging effect. We next implemented a competitive magneto-electrochemical sensor (cMES) optimized for AA detection directly from plasma. The developed platform could detect AA directly from small volumes of plasma (0.5 µL) within 1.5 hour. cMES assays confirmed a strong correlation between AA levels and anti-aging effect: AA levels, while decreasing with aging, increased in the plasma-treated aged mice which also showed improved learning and memory performance. The cMES platform will empower both pre- and clinical anti-aging research by enabling minimally invasive, longitudinal treatment surveillance; these capacities will accelerate the development of anti-aging therapies, improving the quality of individual lives.
ISSN:1838-7640
1838-7640
DOI:10.7150/thno.30422