Expanding the base editing scope in rice by using Cas9 variants

Summary Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that...

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Bibliographic Details
Published in:Plant biotechnology journal 2019-02, Vol.17 (2), p.499-504
Main Authors: Hua, Kai, Tao, Xiaoping, Zhu, Jian‐Kang
Format: Article
Language:English
Subjects:
Adenine
adenine base editor
base editing
Cas9 variants
CRISPR-Associated Protein 9 - genetics
CRISPR-Associated Protein 9 - metabolism
CRISPR-Cas Systems
Cytosine
cytosine base editor
Editing
Gene Editing
Genome editing
Genome, Plant - genetics
Genomes
Genomics
Homology
Oryza - genetics
Plant breeding
Pyrimidines
Rice
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
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Description
Summary:Summary Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.12993