Genome sequence and genetic transformation of a widely distributed and cultivated poplar

Summary Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation ti...

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Bibliographic Details
Published in:Plant biotechnology journal 2019-02, Vol.17 (2), p.451-460
Main Authors: Ma, Jianchao, Wan, Dongshi, Duan, Bingbing, Bai, Xiaotao, Bai, Qiuxian, Chen, Ningning, Ma, Tao
Format: Article
Language:English
Subjects:
Analysis
Annotations
Breeding
Breeding methods
comparative genomics
Disease resistance
Gene families
Genes
Genetic transformation
Genome, Plant - genetics
Genomes
Genomic analysis
Genomics
Molecular Sequence Annotation
Nucleotide sequence
Poplar
Populus - genetics
Populus alba
Populus bolleana
Proteins
Sequence Analysis, DNA
Species
transformation efficiency
Transformation, Genetic
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Summary:Summary Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein‐coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.12989