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Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels
Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen sa...
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Published in: | International journal of molecular sciences 2019-01, Vol.20 (1), p.203 |
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description | Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two groups: control (
= 10, ROS < 102.2 RLU/s/10⁶ sperm) and ROS+ (
= 10, ROS > 102.2 RLU/s/10⁶ sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB). A total of 44 and 371 DEPs were identified between the study groups in the seminal plasma and spermatozoa, respectively. The identified DEPs were primarily involved in oxidoreductase, endopeptidase inhibitor, and antioxidant activities. We validated by WB the underexpression of NADH:ubiquinone oxidoreductase core subunit S1 (
= 0.01), as well as the overexpression of superoxide dismutase 1 (
= 0.03) and peroxiredoxin 4 (
= 0.04) in spermatozoa of ROS+ group. Our data suggest that fertile men with high ROS levels possess an effective antioxidant defense system that protects sperm proteins, as well as an active proteasomal system for degradation of defective proteins. |
doi_str_mv | 10.3390/ijms20010203 |
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= 10, ROS < 102.2 RLU/s/10⁶ sperm) and ROS+ (
= 10, ROS > 102.2 RLU/s/10⁶ sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB). A total of 44 and 371 DEPs were identified between the study groups in the seminal plasma and spermatozoa, respectively. The identified DEPs were primarily involved in oxidoreductase, endopeptidase inhibitor, and antioxidant activities. We validated by WB the underexpression of NADH:ubiquinone oxidoreductase core subunit S1 (
= 0.01), as well as the overexpression of superoxide dismutase 1 (
= 0.03) and peroxiredoxin 4 (
= 0.04) in spermatozoa of ROS+ group. Our data suggest that fertile men with high ROS levels possess an effective antioxidant defense system that protects sperm proteins, as well as an active proteasomal system for degradation of defective proteins.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20010203</identifier><identifier>PMID: 30626014</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antioxidants ; Antioxidants - metabolism ; Biological activity ; Chromatin ; Cytokines ; Deoxyribonucleic acid ; DNA ; DNA fragmentation ; Fertility ; Fragmentation ; Genetic screening ; Genetic testing ; Humans ; Infertility ; Male ; Molecular Sequence Annotation ; Oxidative Stress ; Physiology ; Proteasomes ; Protein expression ; Protein folding ; Proteins ; Proteomics - methods ; Reactive Oxygen Species - metabolism ; Regulation ; Reproductive technologies ; Semen ; Semen - metabolism ; Sperm ; Spermatozoa - metabolism ; Stress response ; Tumor necrosis factor-TNF</subject><ispartof>International journal of molecular sciences, 2019-01, Vol.20 (1), p.203</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-64a6a0b8d4df33d007bd0202dbb75672fbd97f352249c54394495b2b5c3bd9cc3</citedby><cites>FETCH-LOGICAL-c412t-64a6a0b8d4df33d007bd0202dbb75672fbd97f352249c54394495b2b5c3bd9cc3</cites><orcidid>0000-0001-7574-1880 ; 0000-0002-2969-0071 ; 0000-0003-0585-1026 ; 0000-0002-2336-8006</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2331891978/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2331891978?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25728,27898,27899,36986,36987,44563,53763,53765,75093</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30626014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dias, Tânia R</creatorcontrib><creatorcontrib>Samanta, Luna</creatorcontrib><creatorcontrib>Agarwal, Ashok</creatorcontrib><creatorcontrib>Pushparaj, Peter N</creatorcontrib><creatorcontrib>Panner Selvam, Manesh Kumar</creatorcontrib><creatorcontrib>Sharma, Rakesh</creatorcontrib><title>Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two groups: control (
= 10, ROS < 102.2 RLU/s/10⁶ sperm) and ROS+ (
= 10, ROS > 102.2 RLU/s/10⁶ sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB). A total of 44 and 371 DEPs were identified between the study groups in the seminal plasma and spermatozoa, respectively. The identified DEPs were primarily involved in oxidoreductase, endopeptidase inhibitor, and antioxidant activities. We validated by WB the underexpression of NADH:ubiquinone oxidoreductase core subunit S1 (
= 0.01), as well as the overexpression of superoxide dismutase 1 (
= 0.03) and peroxiredoxin 4 (
= 0.04) in spermatozoa of ROS+ group. Our data suggest that fertile men with high ROS levels possess an effective antioxidant defense system that protects sperm proteins, as well as an active proteasomal system for degradation of defective proteins.</description><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Biological activity</subject><subject>Chromatin</subject><subject>Cytokines</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fragmentation</subject><subject>Fertility</subject><subject>Fragmentation</subject><subject>Genetic screening</subject><subject>Genetic testing</subject><subject>Humans</subject><subject>Infertility</subject><subject>Male</subject><subject>Molecular Sequence Annotation</subject><subject>Oxidative Stress</subject><subject>Physiology</subject><subject>Proteasomes</subject><subject>Protein expression</subject><subject>Protein folding</subject><subject>Proteins</subject><subject>Proteomics - methods</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Regulation</subject><subject>Reproductive technologies</subject><subject>Semen</subject><subject>Semen - metabolism</subject><subject>Sperm</subject><subject>Spermatozoa - metabolism</subject><subject>Stress response</subject><subject>Tumor necrosis factor-TNF</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkU1vEzEQhi0EoqXlxhlZ4sKhaf21u_EFKWoprZSqqClny2vPJo527WB7A_0f_GAcWqrAyda8jx7PeBB6R8kp55KcufWQGCGUMMJfoEMqGJsQUjcv9-4H6E1Ka0IYZ5V8jQ44qVlNqDhEv77GkCEMzuCFW3qdxwgJ38EWdI8vXNdBBG9KyXm8yCXbhWkTfIITPPPZhZ_Oap_xBXRQilh7i_84dQpDccxMdluXH3aCS4jZ9YBvwOMfLq_wlVuu8AIG5wt5d7vA8_Jwn47Rq073Cd4-nUfo2-Xn-_Oryfz2y_X5bD4xgrI8qYWuNWmnVtiOc0tI09ryC8y2bVPVDetaK5uOV4wJaSrBpRCyallbGV4SY_gR-vTo3YztANaAz1H3ahPdoOODCtqpfxPvVmoZtqrmvGFTWQQfnwQxfB8hZTW4ZKDvtYcwJsVoIzklpYOCfvgPXYcxlrkLxTmdSiqbaaFOHikTQ0oRuudmKFG7dav9dRf8_f4Az_Df_fLftqmoNw</recordid><startdate>20190108</startdate><enddate>20190108</enddate><creator>Dias, Tânia R</creator><creator>Samanta, Luna</creator><creator>Agarwal, Ashok</creator><creator>Pushparaj, Peter N</creator><creator>Panner Selvam, Manesh Kumar</creator><creator>Sharma, Rakesh</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7574-1880</orcidid><orcidid>https://orcid.org/0000-0002-2969-0071</orcidid><orcidid>https://orcid.org/0000-0003-0585-1026</orcidid><orcidid>https://orcid.org/0000-0002-2336-8006</orcidid></search><sort><creationdate>20190108</creationdate><title>Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels</title><author>Dias, Tânia R ; 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However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two groups: control (
= 10, ROS < 102.2 RLU/s/10⁶ sperm) and ROS+ (
= 10, ROS > 102.2 RLU/s/10⁶ sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB). A total of 44 and 371 DEPs were identified between the study groups in the seminal plasma and spermatozoa, respectively. The identified DEPs were primarily involved in oxidoreductase, endopeptidase inhibitor, and antioxidant activities. We validated by WB the underexpression of NADH:ubiquinone oxidoreductase core subunit S1 (
= 0.01), as well as the overexpression of superoxide dismutase 1 (
= 0.03) and peroxiredoxin 4 (
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subjects | Antioxidants Antioxidants - metabolism Biological activity Chromatin Cytokines Deoxyribonucleic acid DNA DNA fragmentation Fertility Fragmentation Genetic screening Genetic testing Humans Infertility Male Molecular Sequence Annotation Oxidative Stress Physiology Proteasomes Protein expression Protein folding Proteins Proteomics - methods Reactive Oxygen Species - metabolism Regulation Reproductive technologies Semen Semen - metabolism Sperm Spermatozoa - metabolism Stress response Tumor necrosis factor-TNF |
title | Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels |
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