Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells

The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the react...

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Bibliographic Details
Published in:Oncology letters 2019-02, Vol.17 (2), p.1581-1588
Main Authors: Chen, Wen-Xue, Li, Jin-Gao, Wan, Xiang-Hui, Zou, Xue-Sen, Qi, Shu-Yi, Zhang, Yu-Qing, Weng, Qiu-Min, Li, Jun-Yu, Xiong, Wen-Min, Xie, Chen, Cheng, Wei-Liang
Format: Article
Language:English
Subjects:
Biotin
Breast cancer
Cancer
Cancer cells
Cancer research
Care and treatment
Cell adhesion & migration
Design
Diagnosis
Efficiency
Experiments
Immunoglobulins
Innovations
Metastasis
Methods
Microfluidics
Nasopharyngeal cancer
Oncology
Radiation therapy
Throat cancer
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Summary:The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the reaction chamber (consisting of columns) using a biotin-avidin system. Cell suspension was introduced into the reaction chamber to capture NPC cells. NPC cells were subsequently eluted, and the capture rate of the cells was calculated. The modified aptamer-bound microfluidic chip was able to capture NPC cells with a capture rate of ~90%. The modified aptamer-bound microfluidic chip has a wide range of potential applications for the diagnosis of NPC.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2018.9771