miR-146a-5p targets interleukin-1 receptor-associated kinase 1 to inhibit the growth, migration, and invasion of breast cancer cells

MicroRNAs (miRNAs) are associated with the formation and progression of many types of cancers. In the present study, the aim was to elucidate the involvement of miR-146a-5p in the regulation of human breast cancer (BC) cell growth and invasion, as well as the mechanisms underlying its effects. Rever...

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Bibliographic Details
Published in:Oncology letters 2019-02, Vol.17 (2), p.1573-1580
Main Authors: Long, Jing-Pei, Dong, Li-Feng, Chen, Fang-Fang, Fan, Yang-Fan
Format: Article
Language:English
Subjects:
Apoptosis
Brain cancer
Breast cancer
Cancer cells
Cancer research
Care and treatment
Cell adhesion & migration
Cell growth
Cytokines
Development and progression
Gene expression
Genetic aspects
Health aspects
Innovations
Interleukins
Liver cancer
Lung cancer
Metastasis
MicroRNA
MicroRNAs
Molecular targeted therapy
Oncology
Polymerase chain reaction
Studies
Tumors
Womens health
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Summary:MicroRNAs (miRNAs) are associated with the formation and progression of many types of cancers. In the present study, the aim was to elucidate the involvement of miR-146a-5p in the regulation of human breast cancer (BC) cell growth and invasion, as well as the mechanisms underlying its effects. Reverse transcription-quantitative polymerase chain reaction results revealed that miR-146a-5p was markedly downregulated in BC tissues relative to those of adjacent normal tissues. miR-146a-5p expression was also markedly downregulated in BC cells. Overexpression of miR-146a-5p significantly suppressed the proliferation, invasion and migration of BC MDA-MB-453 and MCF7 cells. Furthermore, the results indicated that miR-146a-5p downregulated the expression of interleukin-1 receptor-associated kinase 1 (IRAK1) by directly binding to its 3'-untranslated region in BC cells. Furthermore, IRAK1 expression was observed to be markedly upregulated and inversely correlated with miR-146a-5p expression in BC tissues. Mechanical studies indicated that restoring IRAK1 expression reversed the miR-146a-5p-induced inhibitory effects on proliferation and invasion of BC cells. In conclusion, miR-146a-5p may act as a tumor suppressor in BC by directly targeting IRAK1. These results highlighted the potential of miR-146a-5p as a novel therapeutic target for the treatment of BC.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2018.9769