QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley

Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resu...

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Bibliographic Details
Published in:Breeding Science 2018, Vol.68(5), pp.561-570
Main Authors: Kodama, Asuka, Narita, Ryouhei, Yamaguchi, Makoto, Hisano, Hiroshi, Adachi, Shunsuke, Takagi, Hiroki, Ookawa, Taiichiro, Sato, Kazuhiro, Hirasawa, Tadashi
Format: Article
Language:English
Subjects:
Abiotic stress
Artificial pollination
Barley
composite interval mapping
Crop yield
exome QTL-seq
F-box protein
Fertility
Food production
Gene mapping
Grain
grain fertility
Hordeum vulgare
Inbreeding
Male sterility
Mapping
Pollen
pollen sterility
Pollination
Proteins
Quantitative trait loci
quantitative trait locus
Research Paper
Salinity tolerance
Salt
salt stress
Salts
Seedlings
Sodium chloride
Stress
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Summary:Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.18082