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Purification of a ribosome-inactivating protein with antioxidation and root developer potencies from Celosia plumosa
Considering Celosia plumosa as a potent antiviral plant, the attempt was made to determine, purify and characterize its proteinaceous antiviral elements against tobacco mosaic virus hypersensitive response on Nicotiana glutinosa . By using 60% ammonium sulphate-precipitation, FPLC-based anion and ca...
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Published in: | Physiology and molecular biology of plants 2019-01, Vol.25 (1), p.243-251 |
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Main Author: | |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Considering
Celosia plumosa
as a potent antiviral plant, the attempt was made to determine, purify and characterize its proteinaceous antiviral elements against tobacco mosaic virus hypersensitive response on
Nicotiana glutinosa
. By using 60% ammonium sulphate-precipitation, FPLC-based anion and cation-exchange chromatography in 10 and 50 mM NaCl, size-exclusion chromatography in 50 mM NaCl and SDS–PAGE 10%, a 25 kD antiviral protein with ribosome-inactivating/28S rRNase ability was purified from the leaves of
C
.
plumosa
at vegetative growth stage. The purified protein showed FRAP-based antioxidant activity in vitro and caused 1.7-fold and 1.4-fold increases in the growth rate of root system upon carborundum-based application on the root growth medium of
N
.
glutinosa
. The present work reports an antiviral protein with ribosome-inactivating, antioxidation and root developer potencies in
C. plumosa
as an edible or ornamental plant that may be useful in health and agricultural biotechnology in the future. |
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ISSN: | 0971-5894 0974-0430 |
DOI: | 10.1007/s12298-018-0577-5 |