Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers

Immobilization antigens (i-antigens) are surface membrane proteins that are widely recognized to be the ideal candidates as vaccines antigens for immunization against Cryptocaryon irritans . In this study, we cloned a putative i-antigen gene from C. irritans , which was expressed in all three stages...

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Bibliographic Details
Published in:Scientific reports 2019-01, Vol.9 (1), p.1029-1029, Article 1029
Main Authors: Mo, Ze-Quan, Xu, Shun, Cassidy-Hanley, Donna M., Li, Yan-Wei, Kolbin, Daniel, Fricke, Jennifer M., Li, An-Xing, Clark, Theodore G., Dan, Xue-Ming
Format: Article
Language:English
Subjects:
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Antigens
Cell surface
Cryptocaryon irritans
Epinephelus
Humanities and Social Sciences
Immobilization
Immunization
Immunoglobulin M
Immunoregulation
Membrane proteins
multidisciplinary
Parasites
Protozoa
Science
Science (multidisciplinary)
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Summary:Immobilization antigens (i-antigens) are surface membrane proteins that are widely recognized to be the ideal candidates as vaccines antigens for immunization against Cryptocaryon irritans . In this study, we cloned a putative i-antigen gene from C. irritans , which was expressed in all three stages of the C. irritans life-cycle, and localized primarily to the cell surface. The recombinant GDCI3 i-antigen was expressed and purified using the free-living ciliate, Tetrahymena thermophila as an expression system. The purified recombinant protein was recognized by rabbit anti- C. irritans antiserum and was capable of eliciting immobilizing antibodies in rabbits and fish suggesting that the antigen itself was correctly folded. Following immunization and parasite challenge, groupers vaccinated with, recombinant GDCI3 i-antigen had a 25% cumulative percent survival rate compared to 8.3% for controls. Both non-specific and parasite-specific IgMs were generated in fish following immunization, with the levels of both increasing following challenge. Parasite-specific IgM in mucus could only be elicited after challenge of the GDCI3 i-antigen vaccinated groupers. To our knowledge, this is the first report using the Tetrahymena expression system to generate C. irritans i-antigens and investigate their use for fish vaccination.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-25710-3