Expression of Hypoxia-Inducible Factor 1α (HIF-1α) and Genes of Related Pathways in Altered Gravity

Immune system deterioration in space represents a major risk, which has to be mitigated for exploration-class missions into the solar system. Altered gravitational forces have been shown to regulate adaptation processes in cells of the immune system, which are important for appropriate risk manageme...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-01, Vol.20 (2), p.436
Main Authors: Vogel, Johannes, Thiel, Cora Sandra, Tauber, Svantje, Stockmann, Christian, Gassmann, Max, Ullrich, Oliver
Format: Article
Language:English
Subjects:
Adaptation
CD4 antigen
Cell lines
Cellular manufacture
Experiments
Gene expression
Genes
Gravitational effects
Gravity
Helper cells
Hypoxia
Hypoxia-inducible factor 1
Hypoxia-inducible factor 1a
Immune system
Lymphocytes
Lymphocytes T
Macrophages
Mammals
Metabolism
Monocytes
Physiology
Proteins
Signal transduction
Space missions
Stat3 protein
T cell receptors
Transcription
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immune system deterioration in space represents a major risk, which has to be mitigated for exploration-class missions into the solar system. Altered gravitational forces have been shown to regulate adaptation processes in cells of the immune system, which are important for appropriate risk management, monitoring and development of countermeasures. T lymphocytes and cells of the monocyte-macrophage system are highly migratory cell types that frequently encounter a wide range of oxygen tensions in human tissues and in hypoxic areas, even under homeostatic conditions. Hypoxia-inducible factor 1 and 2 (HIF's) might have an important role in activation of T cells and cells of the monocyte-macrophages system. Thus, we investigated the regulation of HIF-dependent and, therefore, hypoxia-signaling systems in both cell types in altered gravity and performed transcript and protein analysis from parabolic flight and suborbital ballistic rocket experiments. We found that HIF-1α and HIF-1-dependent transcripts were differently regulated in altered gravity, whereas HIF-1α-dependent gene expression adapted after 5 min microgravity. Inter-platform comparisons identified PDK1 as highly responsive to gravitational changes in human U937 myelomonocytic cells and in Jurkat T cells. We suggest HIF-1 as a potential pharmacological target for counteracting immune system deterioration during space flight.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20020436