Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA

This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were use...

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Bibliographic Details
Published in:BioMed research international 2019-01, Vol.2019 (2019), p.1-12
Main Authors: Zheng, Mingyu, Yan, Guanghai, Piao, Hongmei, Jin, Zhehu, Zhu, Lianhua, Wang, Chongyang, Jiang, Jingzhi, Li, Hongmei, Jin, Guangyu, Xian, Zhemin, Li, Liangchang
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Anaphylaxis
Antigens
Cell adhesion & migration
Cytokines
Degranulation
Ear
Enzyme-linked immunosorbent assay
Fc receptors
Histamine
Histology
Hypersensitivity
IL-1β
Immunoglobulin E
Inflammation
Interleukin 13
Interleukin 4
Interleukin 8
Kinases
MAP kinase
Mast cells
Neurosciences
NF-κB protein
Passive cutaneous anaphylaxis
Phosphorylation
Proteins
Rodents
Signal transduction
Signaling
Skin
Syk protein
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways.
ISSN:2314-6133
2314-6141
DOI:10.1155/2019/7823761