Rapid detection of salmonellosis due to Salmonella enterica serovar Typhimurium in Peruvian commercially bred cavies, using indigenous wild bacteriophages

The salmonelloses are among the commonest, most widespread human zoonotic infections. They have generated international networks to attempt their control, since they cause a spectrum of ailments, ranging from inapparent carrier states to full-blown, severe, sometimes deadly diarrheal and systemic di...

Full description

Saved in:
Bibliographic Details
Published in:Germs (Bucureşti) 2018-12, Vol.8 (4), p.178-185
Main Authors: Tamariz, Jesús, Guevara, Víctor, Guerra, Humberto
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Bacterial infections
E coli
Enzyme activation
Health aspects
Infectious diseases
Laboratories
Original
Pathogens
Phages
Salmonella
Salmonellosis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The salmonelloses are among the commonest, most widespread human zoonotic infections. They have generated international networks to attempt their control, since they cause a spectrum of ailments, ranging from inapparent carrier states to full-blown, severe, sometimes deadly diarrheal and systemic disease. Rapid diagnosis is needed for a number of reasons. The aim of this study was to standardize and validate a phage amplification test for the identification of salmonellosis to be applied to infections of . Native bacteriophages were isolated from infected cavies and environmental residues from commercial cavy-breeding facilities. serovar Typhimurium ATCC 14028 was used to detect, isolate and propagate the bacteriophages, and to standardize a phage amplification assay to detect Typhimurium from rectal swabs of cavies. The phage amplification assay was tested using 2 antiviral agents, MgSO ·7H O (MAS) and pomegranate rind extract (PRE) plus ferrous sulfate (PRE-FeSO ). The final assay format chosen used PRE-FeSO and allowed detection of Typhimurium in 90 min from culture, 5 h from clinical samples, with a limit of detection at 10 pfu; sensitivity was 98.2%, specificity 98%, negative predictive value (NPV) 96.1%, and positive predictive value (PPV) 99.1%. Bacteriophage amplification is therefore an appropriate, fast procedure for detection of this pathogen in clinical samples.
ISSN:2248-2997
2248-2997
DOI:10.18683/germs.2018.1144