Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms

The present study aimed to explore the therapeutic effect and underlying mechanism of epidermal growth factor (EGF) on the wound healing of diabetic foot ulcers (DFU). A total of 48 rabbits with DFU were randomly divided into 2 groups, comprising the treatment and control groups. Full-thickness skin...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2019-03, Vol.17 (3), p.1643-1648
Main Authors: Zhang, Juanzi, Hu, Wenxian, Diao, Qixian, Wang, Zhaowei, Miao, Jin, Chen, Xiao, Xue, Zhijun
Format: Article
Language:English
Subjects:
Animals
Biotechnology
Care and treatment
Cytokines
Development and progression
Diabetes
Diabetic foot
Epidermal growth factor
Epidermal growth factors
Fibroblasts
Foot diseases
Foot ulcer
Gangrene
Gene expression
Genetic aspects
Health aspects
Leg ulcers
Medical research
Medical technology
Messenger RNA
Pathogens
Proteins
Rabbits
RNA
Signal transduction
Skin
Studies
Wound care
Wound healing
Wounds
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Summary:The present study aimed to explore the therapeutic effect and underlying mechanism of epidermal growth factor (EGF) on the wound healing of diabetic foot ulcers (DFU). A total of 48 rabbits with DFU were randomly divided into 2 groups, comprising the treatment and control groups. Full-thickness skin (10×10 mm) was excised from the thigh of each rabbit. The wounds in the treatment group were treated with 100 mg/l EGF once a day for 1 month. The control group received no treatment. At 20 days following treatment, new granulation tissues that formed beyond the edge of the wound were collected for subsequent analysis. Tissues from rabbits in the treatment group produced a greater number of fibroblasts, which exhibited a fibroblastic morphology when compared with those in the control group. In the treatment group, a larger number of these fibroblasts were observed as clusters, and there were numerous blood vessels when compared with the control group. The fibroblasts in the control group exhibited an irregular morphology, contained fewer organelles and the surrounding collagenous fibers were sparse. These fibroblasts also demonstrated a disordered arrangement and it was revealed that the wound healed at a slower rate compared with the treatment group. Endogenous EGF mRNA detection revealed that there was a significant difference (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2018.7133