Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection

Cx43 has been documented to be involved in ischemic preconditioning (IPC). However, the participation of Cx43-formed hemichannels in IPC and the potential underlying mechanisms remain unclear. The present study focused on cardiomyocytes' volume regulation during IPC to investigate the role of h...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2019-03, Vol.17 (3), p.1847-1854
Main Authors: Wang, Wenying, Zheng, Dedong, Li, Huiya, Huang, Jinhua, Chen, Huijun, Ying, Teng, Fang, Jun, Luo, Yukun
Format: Article
Language:English
Subjects:
Apoptosis
Cardiomyocytes
Cardiovascular diseases
Cell culture
Edema
Genetic aspects
Genetic regulation
Health aspects
Heart cells
Ischemia
Microscopy
Morphology
Mortality
Prevention
Proteins
Rodents
Studies
Transport proteins
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Summary:Cx43 has been documented to be involved in ischemic preconditioning (IPC). However, the participation of Cx43-formed hemichannels in IPC and the potential underlying mechanisms remain unclear. The present study focused on cardiomyocytes' volume regulation during IPC to investigate the role of hemichannels in the IPC-induced cardioprotection. In the study, mice cardiomyocytes were respectively treated with a hemichannel blocker, octanol or 18a-Glycyrrhizic acid (18a-GA), and a Cx43-silenced lentivirus. They were subsequently cultured in hypotonic solution to simulate ischemic reperfusion (SIR) and systemic ischemic preconditioning (SIP). Cell morphology and volumetric (area) change were detected by inverted microscopy at 30 min following the addition of hypotonic solution. Cardiomyocyte mortality was assessed by trypan blue stain assay. The analyses revealed that regardless of the treatments, hypotonic solution aggravated cell edema: Compared with the initial condition (the moment before the solution addition, 0 min), the volumetric area increased significantly 30 min later (for hypotonic+DMSO, 5,050±1,511 vs. 3,464±723 µm ; for hypotonic+scramble lentiviral vector, 5,517±1,128 vs. 2,331±536 µm ; P0.05 for all). Notably, results indicated that the SIP group had lower mortality rates compared with its SIR counterpart; the hypotonic+octanol, hypotonic+18a-GA, and hypotonic+Cx43-silenced group showed markedly-declined mortality when compared with their respective control groups (respectively, 35.70±1.02, 30.76±2.20 vs. 53.58±2.14%; 30.89±2.37 vs. 54.12±2.55%; P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2018.7127