Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest, anti-angiogenesis and induction of apoptosis

• Published in: Scientific reports 2019-02, Vol.9 (1), p.1514, Article 1514
• Main Authors: Razak, Nursyamirah Abd, Abu, Nadiah, Ho, Wan Yong, Zamberi, Nur Rizi, Tan, Sheau Wei, Alitheen, Noorjahan Banu, Long, Kamariah, Yeap, Swee Keong
• Format: Article
• Language: English
• Subjects: 13/2; 13/31; 14/63; 38/77; 38/79; 631/154/309/2144; 631/67/1347; 631/80/82/23; 82/1; AKT protein; Angiogenesis; Aorta; Apoptosis; Blood vessels; Boyden chamber; Breast cancer; Breast carcinoma; Caspase; Caspase-8; Caspase-9; Cell cycle; Cell migration; Cell proliferation; Cytochrome c; Cytotoxicity; DIABLO protein; Gene expression; Humanities and Social Sciences; multidisciplinary; Science; Science (multidisciplinary); Wound healing
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-37796-w

Eupatorin has been reported with in vitro cytotoxic effect on several human cancer cells. However, reports on the mode of action and detail mechanism of eupatorin in vitro in breast cancer disease are limited. Hence, eupatorin’s effect on the human breast carcinoma cell line MCF-7 and MDA-MB-231 was investigated. MTT assay showed that eupatorin had cytotoxic effects on MCF-7 and MDA-MB-231 cells but was non-toxic to the normal cells of MCF-10a in a time-dose dependent manner. At 24 h, the eupatorin showed mild cytotoxicity on both MCF-7 and MDA-MB-231 cells with IC 50 values higher than 20 μg/mL. After 48 h, eupatorin at 5 μg/mL inhibited the proliferation of MCF-7 and MDA-MB-231 cells by 50% while the IC 50 of MCF-10a was significantly (p