Factorial design-assisted supercritical carbon-dioxide extraction of cytotoxic active principles from Carica papaya leaf juice

The aims of this study are to investigate the selective cytotoxic activity of supercritical carbon dioxide (scCO 2 )-extracted freeze-dried leaf juice (FDLJ) of Carica papaya on squamous cell carcinoma (SCC25) cells, and to delineate the best small scale extraction parameters allowing maximal extrac...

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Bibliographic Details
Published in:Scientific reports 2019-02, Vol.9 (1), p.1716-1716, Article 1716
Main Authors: Khaw, Kooi-Yeong, Parat, Marie-Odile, Shaw, Paul Nicholas, Nguyen, Thao T. T., Pandey, Saurabh, Thurecht, Kristofer J., Falconer, James Robert
Format: Article
Language:English
Subjects:
13/106
631/154/1435
692/4028/67/1813
Bioactive compounds
Carbon dioxide
Carica papaya
Comparative analysis
Cytotoxicity
Experimental design
High pressure
Humanities and Social Sciences
Juices
Leaves
Low temperature
Mass spectrometry
Mass spectroscopy
multidisciplinary
Phytosterols
Science
Science (multidisciplinary)
Squamous cell carcinoma
Vitamins
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Summary:The aims of this study are to investigate the selective cytotoxic activity of supercritical carbon dioxide (scCO 2 )-extracted freeze-dried leaf juice (FDLJ) of Carica papaya on squamous cell carcinoma (SCC25) cells, and to delineate the best small scale extraction parameters allowing maximal extract activity. Using scCO 2 as a solvent, six operating parameters were studied and the supercritical fluid extraction (SFE) process investigated using a factorial design 2 6-2 . The processing values promoting cytotoxic activity towards SCC-25 are: high pressure (250 bar), low temperature (35 °C), extended processing time (180 minutes), as well as a large amount of starting material (5 g). The factorial experimental design successfully identified the key parameters controlling the SFE of molecules cytotoxic to SCC cells from C. papaya juice. This study also validated the extraction method and showed that the SFE yield was reproducible. The chromatographic and mass spectrometric profiles of the scCO 2 extract acquired with high-resolution quadrupole time-of-flight mass spectrometry (LC-QToF-MS) were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds were likely to be mainly vitamins and phytosterols, some of which are documented to be cytotoxic to cancer cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-37171-9