Integrator is a key component of human telomerase RNA biogenesis

Telomeres are special DNA-protein structures that are located at the ends of linear eukaryotic chromosomes. The telomere length determines the proliferation potential of cells. Telomerase is a key component of the telomere length maintenance system. While telomerase is inactive in the majority of so...

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Bibliographic Details
Published in:Scientific reports 2019-02, Vol.9 (1), p.1701-1701, Article 1701
Main Authors: Rubtsova, M. P., Vasilkova, D. P., Moshareva, M. A., Malyavko, A. N., Meerson, M. B., Zatsepin, T. S., Naraykina, Y. V., Beletsky, A. V., Ravin, N. V., Dontsova, O. A.
Format: Article
Language:English
Subjects:
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Cancer
Cell proliferation
Chromosomes
Deoxyribonucleic acid
DNA
DNA-directed RNA polymerase
Humanities and Social Sciences
Immortalization
multidisciplinary
RNA polymerase
RNA processing
RNA-directed DNA polymerase
Science
Science (multidisciplinary)
Somatic cells
Stem cells
Telomerase
Telomerase reverse transcriptase
Telomeres
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Summary:Telomeres are special DNA-protein structures that are located at the ends of linear eukaryotic chromosomes. The telomere length determines the proliferation potential of cells. Telomerase is a key component of the telomere length maintenance system. While telomerase is inactive in the majority of somatic cells, its activity determines the clonogenic potential of stem cells as a resource for tissue and organism regeneration. Reactivation of telomerase occurs during the process of immortalization in the majority of cancer cells. Telomerase is a ribonucleoprotein that contains telomerase reverse transcriptase and telomerase RNA components. The RNA processing mechanism of telomerase involves exosome trimming or degradation of the primary precursor. Recent data provide evidence that the competition between the processing and decay of telomerase RNA may regulate the amount of RNA at the physiological level. We show that termination of human telomerase RNA transcription is dependent on its promoter, which engages with the multisubunit complex Integrator to interact with RNA polymerase II and terminate transcription of the human telomerase RNA gene followed by further processing.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-38297-6