Fibrin glue as a local drug-delivery system for bacteriophage PA5

Fibrin glue has been used clinically for decades in a wide variety of surgical specialties and is now being investigated as a medium for local, prolonged drug delivery. Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or pos...

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Bibliographic Details
Published in:Scientific reports 2019-02, Vol.9 (1), p.2091-2091, Article 2091
Main Authors: Rubalskii, Evgenii, Ruemke, Stefan, Salmoukas, Christina, Aleshkin, Andrey, Bochkareva, Svetlana, Modin, Evgeny, Mashaqi, Bakr, Boyle, Erin C., Boethig, Dietmar, Rubalsky, Maxim, Zulkarneev, Eldar, Kuehn, Christian, Haverich, Axel
Format: Article
Language:English
Subjects:
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45/77
631/326/1321
692/308/153
Antibacterial activity
Antibiotic resistance
Antibiotics
Bacteria
Biocompatibility
Biofilms
Drug delivery
Drug resistance
Fibrin
Humanities and Social Sciences
Medical equipment
Microscopy
multidisciplinary
Phages
R&D
Research & development
Science
Science (multidisciplinary)
Transplants & implants
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Summary:Fibrin glue has been used clinically for decades in a wide variety of surgical specialties and is now being investigated as a medium for local, prolonged drug delivery. Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or postoperatively for deep wounds. However, prolonged local application of antibiotics is often not possible or simply inadequate. Biofilm formation and antibiotic resistance are also major obstacles to antibacterial therapy. In this paper we test the biocompatibility of bacteriophages incorporated within fibrin glue, track the release of bacteriophages from fibrin scaffolds, and measure the antibacterial activity of released bacteriophages. Fibrin glue polymerized in the presence of the PA5 bacteriophage released high titers of bacteriophages during 11 days of incubation in liquid medium. Released PA5 bacteriophages were effective in killing Pseudomonas aeruginosa PA01. Overall, our results show that fibrin glue can be used for sustained delivery of bacteriophages and this strategy holds promise for many antibacterial applications.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-38318-4