Autophagy Activation by Crepidiastrum Denticulatum Extract Attenuates Environmental Pollutant-Induced Damage in Dermal Fibroblasts

Pollution-induced skin damage results in oxidative stress; cellular toxicity; inflammation; and, ultimately, premature skin aging. Previous studies suggest that the activation of autophagy can protect oxidation-induced cellular damage and aging-like changes in skin. In order to develop new anti-poll...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-01, Vol.20 (3), p.517
Main Authors: Yoon, Seok Jeong, Lim, Chae Jin, Chung, Hwa-Jee, Kim, Joo-Hwan, Huh, Yang Hoon, Park, Keedon, Jeong, Sekyoo
Format: Article
Language:English
Subjects:
Aging
Antioxidants
Autophagy
Cadmium
Calcium ions
Calcium signalling
Chloride
Cytotoxicity
Deprivation
Differentiation
Fibroblasts
Immunofluorescence
Industrial development
Investigations
Ion concentration
Keratinocytes
Kinases
Organic chemicals
Oxidants
Oxidative stress
Oxidizing agents
Phagocytosis
Phosphorylation
Pollutants
Protein transport
Proteins
Rapamycin
Retinoic acid
Retinoic acid receptors
Signal transduction
TOR protein
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Summary:Pollution-induced skin damage results in oxidative stress; cellular toxicity; inflammation; and, ultimately, premature skin aging. Previous studies suggest that the activation of autophagy can protect oxidation-induced cellular damage and aging-like changes in skin. In order to develop new anti-pollution ingredients, this study screened various kinds of natural extracts to measure their autophagy activation efficacy in cultured dermal fibroblast. The stimulation of autophagy flux by the selected extracts was further confirmed both by the expression of proteins associated with the autophagy signals and by electron microscope. (CD) extract treated cells showed the highest autophagic vacuole formation in the non-cytotoxic range. The phosphorylation of adenosine monophosphate kinase (AMPK), but not the inhibition of mammalian target of rapamycin (mTOR), was observed by CD-extract treatment. Its anti-pollution effects were further evaluated with model compounds, benzo[a]pyrene (BaP) and cadmium chloride (CdCl₂), and a CD extract treatment resulted in both the protection of cytotoxicity and a reduction of proinflammatory cytokines. These results suggest that the autophagy activators can be a new protection regimen for anti-pollution. Therefore, CD extract can be used for anti-inflammatory and anti-pollution cosmetic ingredients.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20030517