Anthocyanin Degrading and Chlorophyll Accumulation Lead to the Formation of Bicolor Leaf in Ornamental Kale

Ornamental kale is a popular decorative plant. We identified a peculiar bicolor leaf double haploid line, with green margins and red centers. The development of bicolor leaves can be divided into three stages: S1, S2, and S3. To probe the reason for bicolor formation, we analyzed the anthocyanin and...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-01, Vol.20 (3), p.603
Main Authors: Ren, Jie, Liu, Zhiyong, Chen, Weishu, Xu, Hezi, Feng, Hui
Format: Article
Language:English
Subjects:
Abscisic acid
Accumulation
Anthocyanins - metabolism
Biosynthesis
Brassica - genetics
Brassica - metabolism
Brassica - physiology
Chlorophyll
Chlorophyll - metabolism
Flavonoids
Gene expression
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Leaves
Metabolism
Photosynthesis
Pigmentation - genetics
Pigmentation - physiology
Pigments
Plant Leaves - metabolism
Plant Proteins - metabolism
Starch
Transcription factors
Transcriptome - genetics
Transcriptome - physiology
Vegetables
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ornamental kale is a popular decorative plant. We identified a peculiar bicolor leaf double haploid line, with green margins and red centers. The development of bicolor leaves can be divided into three stages: S1, S2, and S3. To probe the reason for bicolor formation, we analyzed the anthocyanin and chlorophyll contents, detected the changes in indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellin 3 (GA3), sugar, and starch contents, and identified the differentially expressed genes (DEGs) using RNA-seq. Results showed that the bicolor leaf phenotype is gradually formed with anthocyanin degrading and chlorophyll accumulation. Anthocyanin content is lower in the green margin (S3_S) than in the red center (S3_C) part at S3. IAA content was positively correlated with anthocyanin content during the bicolor leaf development. During anthocyanin degrading from S1 to S2, ( ) and ( ) were downregulated, while ( ) was upregulated. Two peroxidases, two β-glucosidases ( ), , , ( ), three ( ) transcription factors (TFs), and 15 WRKY DNA-binding protein (WRKY) TFs were downregulated in S3_S S3_C. The bicolor phenotype was mainly linked to anthocyanin degrading and chlorophyll accumulation, and that anthocyanin degrading resulted from reduced anthocyanin biosynthesis and increased anthocyanin degradation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20030603