Single‐Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single‐wavelength excitation and emission for multiplexed cellular imaging is extremely cha...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2018-10, Vol.57 (41), p.13686-13690
Main Authors: Chen, Chi, Ao, Lijiao, Wu, Yu‐Tang, Cifliku, Vjona, Cardoso Dos Santos, Marcelina, Bourrier, Emmanuel, Delbianco, Martina, Parker, David, Zwier, Jurriaan M., Huang, Liang, Hildebrandt, Niko
Format: Article
Language:English
Subjects:
Coding
Communication
Communications
Europium - chemistry
Field of view
Fluorescence
Fluorescence resonance energy transfer
Fluorescence Resonance Energy Transfer - methods
FRET
imaging
Lanthanides
Life Sciences
Medical imaging
Multiplexing
Nanoparticles
Photoluminescence
photoluminescence lifetimes
Photons
Quantum Dots
Silicon dioxide
Terbium - chemistry
Windows (intervals)
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Summary:Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single‐wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Herein, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) that are coated with two different lanthanide complexes (Tb and Eu). FRET from the Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The well‐defined single‐nanoparticle codes were used for live cell imaging and a one‐measurement distinction of four different cells in a single field of view. This single‐color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells. Temporal RGB codes: FRET from two different lanthanide complexes (Tb and Eu) to quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The single‐nanoparticle codes were used for live cell imaging.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201807585