Determination of IgE and IgG reactivity to more than 170 allergen molecules in paper-dried blood spots

• Published in: Journal of allergy and clinical immunology 2019-01, Vol.143 (1), p.437-440
• Main Authors: Garib, Victoria, Rigler, Eva, Gastager, Felix, Campana, Raffaela, Dorofeeva, Yulia, Gattinger, Pia, Zhernov, Yury, Khaitov, Musa, Valenta, Rudolf
• Format: Article
• Language: English
• Subjects: Allergens; Allergens - chemistry; Allergens - immunology; Allergies; Blood; Demographics; Dried Blood Spot Testing - methods; Elution; Experiments; Female; Filter paper; Humans; Hypersensitivity - blood; Hypersensitivity - immunology; Immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; Internet; Laboratories; Male; Proteins; Storage
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2018.08.047

The demographic and clinical characterization of the 17 patients (ie, #1-#18) is presented in Table E2 in this article's Online Repository at www.jacionline.org. [...]136 analyses were performed in triplicates for each condition (ie, immediate recovery and recovery after 2 weeks of storage at 37°C) (see Fig E5 in this article's Online Repository at www.jacionline.org). Table E3 in this article's Online Repository at www.jacionline.org presents for sera from 9 allergic patients the protein contents and recovery of protein after storage for 2 weeks at +37°C, +22°C, +4°C, −20°C. The range of recovery for the 9 sera at the 4 tested conditions was 9% to 18% with the elution procedure applied in our experiments (ie, dried serum, filter paper, 3 punched pieces, 50 μL PBS). Because measurement of allergen-specific IgG to micro-arrayed allergens is also important to study clinically relevant questions such as the induction of allergen-specific IgG in the course of allergen-specific immunotherapy8 or the occurrence of allergen-specific IgG as a sign of natural allergen exposure9 we studied whether allergen-specific IgG can be also recovered from paper-dried serum spots. Because the paper-dried blood spots can be stored at different temperatures (−20°C-+37°C) without losing activity, it should be possible to send them sealed in plastic by envelopes via simple mail from all over the world to laboratories performing micro-array analysis.